Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial.
Recombinant antigen-based antibody assays for the diagnosis and surveillance of lymphatic filariasis - a multicenter trial.
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DOI：
10.1186/1475-2883-3-9
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发表时间：
2004-09-03
期刊：
影响因子：
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通讯作者：
Ottesen, Eric
中科院分区：
文献类型：
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作者：
Lammie, Patrick J;Weil, Gary;Ottesen, Eric
抗丝虫抗体反应的产生是丝虫寄生虫感染的特征。应该可以利用这一事实来开发工具来监测消除淋巴丝虫病（LF）全球计划的进展情况；然而，基于寄生虫提取物的测定受到许多限制，包括寄生虫材料的缺乏、测定标准化的困难以及测定特异性的问题。原则上，基于重组丝虫抗原的检测应解决这些局限性，并为 LF 的诊断和监测提供有用的工具。本多中心研究旨在比较基于重组抗原 Bm14、WbSXP 和 BmR1 的丝虫病抗体检测的性能。编码的血清样本被分发到五个参与的实验室，其中每种抗原的测定平行进行。基于 Bm14、WbSXP 或 BmR1 的检测显示出良好的现场使用灵敏度 (>90%)，并且所有检测均未显示出与非丝虫蠕虫感染者的标本有反应性。讨论了测定的局限性。现在需要精心设计的实地研究来评估采样方法以及抗体测试在 LF 消除计划监测和监督中的应用。
The development of antifilarial antibody responses is a characteristic feature of infection with filarial parasites. It should be possible to exploit this fact to develop tools to monitor the progress of the global program to eliminate lymphatic filariasis (LF); however, assays based on parasite extracts suffer from a number of limitations, including the paucity of parasite material, the difficulty of assay standardization and problems with assay specificity. In principle, assays based on recombinant filarial antigens should address these limitations and provide useful tools for diagnosis and surveillance of LF. The present multicenter study was designed to compare the performance of antibody assays for filariasis based on recombinant antigens Bm14, WbSXP, and BmR1. Coded serum specimens were distributed to five participating laboratories where assays for each antigen were conducted in parallel. Assays based on Bm14, WbSXP, or BmR1 demonstrated good sensitivity (>90%) for field use and none of the assays demonstrated reactivity with specimens from persons with non-filarial helminth infections. Limitations of the assays are discussed. Well-designed field studies are now needed to assess sampling methodology and the application of antibody testing to the monitoring and surveillance of LF elimination programs.