Nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharide alginate synthesis.
Nucleoside diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharide alginate synthesis.
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铜绿假单胞菌的核苷二磷酸激酶：该基因的特征及其在细胞生长和胞外多糖藻酸盐合成中的作用。
DOI：
10.1111/j.1365-2958.1996.tb02538.x
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发表时间：
1996
影响因子：
3.6
通讯作者：
Chakrabarty,AM
中科院分区：
文献类型：
--
作者：
Sundin,GW;Shankar,S;Chugani,SA;Chopade,BA;Kavanaugh-Black,A;Chakrabarty,AM
We report the cloning and determination of the nucleotide sequence of the gene encoding nucleoside diphosphate kinase (Ndk) fromPseudomonas aeruginosa.The amino acid sequence of Ndk was highly homologous with other known bacterial and eukaryotic Ndks (39.9 to 58.3% amino acid identity). We have previously reported thatP. aeruginosastrains with mutations in the genesalgR2andalgR2 algHproduce extremely low levels of Ndk and, as a consequence, are defective in their ability to grow in the presence of Tween 20, a detergent that inhibits a kinase which can substitute for Ndk. Hyperexpression ofndkfrom the clone pGWS95in transin theP. aeruginosa algR2an6 algR2 algHdouble mutant restored Ndk production to levels which equalled or exceeded wild‐type levels and enabled these strains to grow in the presence of Tween 20. Hyperexpression ofndkfrom pGWS95 in theP. aeruginosa algR2mutant also restored alginate production to levels that were approximately 60% of wild type. Nucleoside diphosphate kinase activity was present in both the cytosolic and membrane‐associated fractions ofP. aeruginosa.The cytosolic Ndk was non‐specific in its transfer activity of the terminal phosphate from ATP to other nucleoside diphosphates. However, the membrane form of Ndk was more active in the transfer of the terminal phosphate from ATP to GDP resulting in the predominant formation of GTP. We report in this work that pyruvate kinase and Ndk form a complex which alters the specificity of Ndk substantially to GTP. The significance of GTP in signal transduction
