INFLUENCE OF STARVATION AND REFEEDING ON MUCOSAL SIZE AND EPITHELIAL RENEWAL IN RAT SMALL-INTESTINE
INFLUENCE OF STARVATION AND REFEEDING ON MUCOSAL SIZE AND EPITHELIAL RENEWAL IN RAT SMALL-INTESTINE
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DOI：
10.1002/aja.1001330403
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发表时间：
1972-01-01
影响因子：
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通讯作者：
ALTMANN, GG
中科院分区：
文献类型：
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作者：
ALTMANN, GG
成年雄性大鼠禁食0（对照）、3、5、7天；一组人在六天的饥饿后被喂食一天。组织学样本取自小肠的五个区域。通过测量绒毛和隐窝切片中上皮细胞的数量以及隐窝切片中有丝分裂象的数量来估计绒毛、隐窝和有丝分裂池的大小。进一步的研究使用秋水仙碱估算有丝分裂时间，用甲氨蝶呤抑制有丝分裂。这三个参数在饥饿期间逐渐降低；绒毛大小的减少在十二指肠最为明显，向远处逐渐减少。再喂增加了各区域的有丝分裂池；隐窝大小未增加，绒毛大小仅在十二指肠和空肠略有增加。当再喂与有丝分裂抑制相结合时，隐窝细胞群减少30-40%，绒毛大小不变；因此，更新似乎通过隐窝细胞迁移到绒毛继续进行。隐窝中的有丝分裂用于成肠上皮细胞的更新。在饥饿的各个阶段，计算的上皮周转时间都比正常的长，并且相似。重新进食似乎能刺激恢复活力。由于绒毛大小的变化在一定程度上独立于有丝分裂活动，因此表明了控制绒毛大小的单独机制的参与。
Adult male rats were fasted for 0 (controls), three, five and seven days; a group was refed for one day after six days of starvation. Histological samples were taken from five regions along the length of the small intestine. The sizes of the villi, crypts and mitotic pool were estimated by measuring the number of epithelial cells per villus and crypt section and the number of mitotic figures per crypt section. Additional studies were carried out using colchicine for estimating mitotic time and methotrexate for inhibiting mitosis.All three parameters decreased progressively during starvation; the decrease in villus size was most pronounced in the duodenum and gradually less distalward. Refeeding increased the mitotic pool in every region; crypt size did not increase and villus size increased slightly in duodenum and jejunum only. When refeeding was combined with mitotic inhibition, the cell population of the crypts became depleted by 30–40% without change in villus size; thus, renewal appeared to continue by crypt cells migrating to the villi.Mitosis in the crypts is used for epithelial renewal in the adult intestine. The calculated turnover time of the epithelium was longer than normal and similar in every stage of starvation. Refeeding appeared to stimulate renewal. Since villus size changed somewhat independently from mitotic activity, the involvement of a separate mechanism controlling villus size was indicated.