p57Kip2 is expressed in quiescent mouse bone marrow side population cells
p57Kip2 is expressed in quiescent mouse bone marrow side population cells
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DOI：
10.1016/j.bbrc.2005.09.008
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发表时间：
2005-11-11
影响因子：
3.1
通讯作者：
Okano, T
中科院分区：
文献类型：
--
作者：
Umemoto, T;Yamato, M;Okano, T
造血干细胞可以通过侧群（SP）表型准确鉴定。以前已经表明，造血干细胞是细胞周期阻滞的，但所涉及的机制目前知之甚少。在本研究中，从定量实时RT-PCR的结果表明，虽然SP细胞的各种细胞周期蛋白和细胞周期蛋白依赖性激酶的表达增加，细胞周期蛋白依赖性激酶抑制剂，特别是p57（Kip 2）的表达增加，是负责观察到的细胞周期停滞。此外，c-kit（+/）/Sca-（1+）/Lineage（-）SP（KSL-SP）细胞的基因表达分析表明，与SP和非SP细胞相比，只有p57（Kip 2）显示出更高的表达。此外，免疫染色还显示KSL-SP SP细胞中显著更高的蛋白表达。这些结果表明，骨髓SP细胞在G 0/G1期的维持可能受到p57（Kip 2）的精心控制。(c)2005年爱思唯尔公司All rights reserved.
Hematopoietic stem cells can be accurately identified by the side population (SP) phenotype. It has been previously shown that hematopoietic stem cells are cell cycle arrested, but the mechanisms involved are currently poorly understood. In the present study, results from quantitative real-time RT-PCR show that while SP cells have increased expression of various cyclins and cyclin-dependent kinases, the increased expression of cyclin-dependent kinase inhibitors, in particular p57(Kip2), is responsible for the observed cell cycle arrest. In addition, gene expression analysis of c-kit(+/)/Sca-(1+)/Lineage(-)SP (KSL-SP) cells demonstrates that only p57(Kip2) shows both higher expression compared to both SP and non-SP cells. Furthermore, immunostaining also demonstrates significantly higher protein expression in KSL-SP SP cells. These results demonstrate that the maintenance of bone marrow SP cells in G0/G1 may be carefully controlled by p57(Kip2). (c) 2005 Elsevier Inc. All rights reserved.