Microtubules in the formation and development of the primary mesenchyme in Arbacia punctulata. II. An experimental analysis of their role in development and maintenance of cell shape.
Microtubules in the formation and development of the primary mesenchyme in Arbacia punctulata. II. An experimental analysis of their role in development and maintenance of cell shape.
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DOI：
10.1083/jcb.41.1.227
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发表时间：
1969-04
期刊：
影响因子：
--
通讯作者：
Gibbins JR
中科院分区：
文献类型：
--
作者：
Tilney LG;Gibbins JR
为了通过实验验证前一篇论文中提出的观点，即微管在初级间质的形成和分化过程中参与细胞形状发育，我们对胚胎应用了两种影响细胞质微管的试剂：(a) 秋水仙碱和静水压，它们会导致微管分解，(b) D2O 会稳定微管。当第一种试剂应用于海胆原肠胚时，初级间充质的发育停止，微管消失，并且细胞趋于球形化。 D2O 发育也停止，但肾小管似乎“冻结”，并且细胞不对称性持续不变。这些药物似乎主要通过干扰微管顺序分解和/或重新组装成新模式来阻碍发育。因此，微管似乎对细胞形态的发育有影响。另一方面，通过仔细分析这些药物和其他药物对细胞内和细胞外因子的作用，我们得出结论，微管对于维持分化组织中的细胞形状作用相当小。
To experimentally test the suggestion made in the preceding paper that the microtubules are involved in cell shape development during the formation and differentiation of the primary mesenchyme, we applied to the embryos two types of agents which affect cytoplasmic microtubules: (a) colchicine and hydrostatic pressure, which cause the microtubules to disassemble, and (b) D2O, which tends to stabilize them. When the first type of agent is applied to sea urchin gastrulae, the development of the primary mesenchyme ceases, the microtubules disappear, and the cells tend to spherulate. With D2O development also ceases, but the tubules appear "frozen," and the cell asymmetries persist unaltered. These agents appear to block development by primarily interfering with the sequential disassembly and/or reassembly of microtubules into new patterns. The microtubules, therefore, appear to be influential in the development of cell form. On the other hand through a careful analysis of the action of these agents and others on both intra- and extracellular factors, we concluded that the microtubules do rather little for the maintenance of cell shape in differentiated tissues.