Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis
Enhancement of cold and salt tolerance of Arabidopsis by transgenic expression of the S-adenosylmethionine decarboxylase gene from Leymus chinensis
复制标题
转基因表达羊草S-腺苷甲硫氨酸脱羧酶基因增强拟南芥的耐冷和耐盐能力
DOI：
10.1016/j.jplph.2016.12.014
复制
发表时间：
2017-04-01
影响因子：
4.3
通讯作者：
Liu, Gongshe
中科院分区：
文献类型：
--
作者：
Liu, Zhujiang;Liu, Panpan;Liu, Gongshe
羊草是欧亚草原上天然分布的一种重要多年生牧草。然而，人们对它适应极端环境条件的分子机制知之甚少。基于L.从L. chinensis冷处理序列数据库中克隆了一个高效表达的S-腺苷甲硫氨酸脱羧酶基因（LcSAMDCI）。chinensis。基因结构分析表明，LcSAMDCI的mRNA序列中含有2个内含子和3个外显子，并含有3个不重叠的开放阅读框。1小时的冷暴露引起LcSAMDCI的显着上调，而脱落酸（阿坝），盐和渗透胁迫轻微诱导其表达。不同组织中的基因表达分析表明，LcSAMDCI普遍表达，在幼穗和根茎中的表达水平较高。LcSAMDCI的主要ORF在转基因拟南芥中的过表达促进了相对于野生型拟南芥对冷和盐胁迫的耐受性的增加。多胺、脯氨酸和叶绿素的含量在转基因拟南芥中显著增加，多胺中精胺的含量在低温胁迫下比盐胁迫下增加更多。这些结果表明，LcSAMDCI是冷胁迫诱导的，并可能影响多胺的产生，而多胺的产生与L. chinensis。此外，LcSAMDCI的转基因表达可用于提高作物的非生物抗性。(C)2017 Elsevier GmbH. All rights reserved.
Leymus chinensis is an important perennial forage grass natively distributed in the Eurasian Steppe. However, little is known about the molecular mechanism of its adaptation to extreme environmental conditions. Based on L. chinensis cold-treated sequence database, a highly expressed S-adenosylmethionine decarboxylase gene (LcSAMDCI) was isolated from L. chinensis. Gene structure analysis showed that LcSAMDCI has two introns and three exons as well as three non-overlapping ORFs in its mRNA sequence. One hour of cold exposure caused a significant up-regulation of LcSAMDCI, while abscisic acid (ABA), salt, and osmotic stresses slightly induced its expression. Analysis of gene expression in different tissues showed that LcSAMDCI was expressed ubiquitously, with higher levels in the young spike and rhizome. Overexpression of the main ORF of LcSAMDCI in transgenic Arabidopsis promoted increased tolerance to cold and salt stress relative to wild type Arabidopsis. The concentration of polyamines, proline, and chlorophyll was significantly higher in transgenic Arabidopsis, and spermine of polyamines increased more under cold than under salt stress. These results suggest that LcSAMDCI was induced in response to cold and could influence the production of polyamines involved in stress tolerance of L. chinensis. Moreover, transgenic expression of LcSAMDCI could be used to improve the abiotic resistance of crops. (C) 2017 Elsevier GmbH. All rights reserved.