Differential time-course of the increase of antioxidant thiol-defenses in the acute phase after spinal cord injury in rats
Differential time-course of the increase of antioxidant thiol-defenses in the acute phase after spinal cord injury in rats
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DOI：
10.1016/j.neulet.2009.01.020
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发表时间：
2009-03-06
影响因子：
2.5
通讯作者：
Rios, Camilo
中科院分区：
文献类型：
--
作者：
Diaz-Ruiz, Araceli;Alcaraz-Zubeldia, Mireya;Rios, Camilo
脊髓损伤（SCI）是一个世界性的健康问题。创伤性损伤后，脊髓组织启动一系列自毁机制，称为继发性损伤。 SCI后损伤的主要机制是兴奋性毒性、自由基过量产生、炎症和细胞凋亡。金属硫蛋白 (MT) 和还原型谷胱甘肽 (GSH) 是低分子量、富含半胱氨酸的肽，能够清除自由基。 MT 和 GSH 作为 SCI 后神经保护分子的参与尚不清楚。本研究的目的是描述大鼠SCI后急性期MT和GSH含量以及GSH过氧化物酶（GPx）活性的变化。通过计算机控制装置（纽约大学冲击器）将体重为 200-250 克的雌性 Wistar 大鼠置于脊髓挫伤模型中。接受椎板切除术的大鼠用作对照组。手术后2、4、12和24小时处死动物。使用原子吸收分光光度法通过银饱和法对 MT 进行定量。 GSH 和 GPx 通过分光光度法测定。结果表明，与假手术组值相比，仅在 4 小时和 24 小时时 SCI 的影响导致 MT 含量增加。同时，发现SCI后4、12和24小时GSH下降。有趣的是，GPx 活性在所有时间点均升高，表明这种酶防御在 SCI 后很快被激活。结果表明，基于硫醇的防御机制 MT 和 GSH 在脊髓组织中差异表达，以应对损伤后的各种损伤过程。 (C) 2009 Elsevier Ireland Ltd. 保留所有权利。
Spinal cord injury (SCI) is a world-wide health problem. After traumatic injury, spinal cord tissue starts a series of self-destructive mechanisms, known as the secondary lesion. The leading mechanisms of damage after SCI are excitotoxicity, free radicals' overproduction, inflammation and apoptosis. Metallothionein (MT) and reduced glutathione (GSH) are low-molecular-weight, cysteine-rich peptides able to scavenge free radicals. MT and GSH participation as neuroprotective molecules after SCI is unknown. The aim of the present study is to describe the changes of MT and GSH contents and GSH peroxidase (GPx) activity in the acute phase after SCI in rats. Female Wistar rats weighing 200-250 g were submitted to spinal cord contusion model, by means of a computer-controlled device (NYU impactor). Rats receiving laminectomy were used as a control group. Animals were killed 2, 4, 12 and 24h after surgery. MT was quantified by the silver-saturation method, using atomic absorption spectrophotometry. GSH and GPx were assayed by spectrophotometry. Results indicate an increased MT content by effect of SCI, only at 4 and 24h, as compared to sham group values. Meanwhile, GSH was found decreased at 4, 12 and 24h after SCI. Interestingly, GPx activity was raised at all time points, indicating that this enzymatic defense is activated soon after SCI. Results suggest that thiol-based defenses, MT and GSH, are differentially expressed by spinal cord tissue to cope with the various processes of damage after lesion. (C) 2009 Elsevier Ireland Ltd. All rights reserved.