Studies on bovine factor X. I. Large-sclae purification of the bovine plasma protein possessing factor X activity.
Studies on bovine factor X. I. Large-sclae purification of the bovine plasma protein possessing factor X activity.
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DOI：
10.1021/bi00852a046
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发表时间：
1968-12
期刊：
影响因子：
2.9
通讯作者：
C. Jackson;T. Johnson;D. Hanahan
中科院分区：
文献类型：
--
作者：
C. Jackson;T. Johnson;D. Hanahan
材料与方法常用无机化学品为试剂级，购自国内主要供应商。硫酸钡，适用于X射线诊断（Merck & Co.，Rahway，N. J.，DEAE-纤维素（Selectacel，20型，1.0-mequiv/g容量，批号1544，Schleicher & Schuell，N. Y.）的情况下进行。在使用前依次用1 n NaOH、蒸馏H2O、1 n HCl、蒸馏H2O洗涤至中性，然后用95%乙醇洗涤，最后用无水乙醇洗涤。使用吸水器真空除去乙醇24-48小时。
Materials and MethodsCommon inorganic chemicals were reagent grade and were purchased from major domestic suppliers. Barium sulfate, suitable for X-ray diagnosis (Merck & Co., Rahway, N. J., lot no. E 39785), was used exclusively.DEAE-cellulose (Selectacel, type 20, 1.0-mequiv/g capacity, lot 1544, Schleicher & Schuell, N. Y.) was washed prior to use successively with 1 n NaOH, dis-tilled H20, 1 n HC1, distilled H20 until neutral, then with 95% ethanol, and finally with absolute ethanol. The ethanol was removed in vacuo using a water aspirator for 24-48 hr.