Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents.
Carbon Dots as a New Class of Diamagnetic Chemical Exchange Saturation Transfer (diaCEST) MRI Contrast Agents.
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DOI：
10.1002/anie.201904722
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发表时间：
2019-06
影响因子：
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通讯作者：
Jia Zhang;Yue Yuan;Minling Gao;Zheng Han;Chengyan Chu;Yuguo Li;P. V. van Zijl;M. Ying;J. Bulte;Guanshu Liu
中科院分区：
文献类型：
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作者：
Jia Zhang;Yue Yuan;Minling Gao;Zheng Han;Chengyan Chu;Yuguo Li;P. V. van Zijl;M. Ying;J. Bulte;Guanshu Liu
虽然碳量子点（C-量子点）已经被广泛地研究了它们的荧光、磷光、电致发光、光电和催化特性，但是它们固有的化学交换饱和转移磁共振成像（CEST MRI）特性是未知的。凭借其亲水性和丰富的可交换质子的羟基，胺，和酰胺锚定在表面上，我们在这里报告的C-点可以适应作为有效的抗磁性CEST（diaCEST）MRI造影剂。作为概念验证演示，用具有或不具有包封的C点的脂质体标记人神经胶质瘤细胞，并植入小鼠脑中。体内CEST MRI能够清楚地区分标记细胞和非标记细胞。目前的研究结果可能会鼓励新的应用程序的C-点在体内成像在深部组织，这是目前不可能使用传统的荧光（近红外）的C-点。
While carbon dots (C-dots) have been extensively investigated pertaining to their fluorescent, phosphorescent, electrochemiluminescent, optoelectronic, and catalytic features, their inherent chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) properties are unknown. By virtue of their hydrophilicity and abundant exchangeable protons of hydroxyl, amine, and amide anchored on the surface, we report here that C-dots can be adapted as effective diamagnetic CEST (diaCEST) MRI contrast agents. As a proof-of-concept demonstration, human glioma cells were labeled with liposomes with or without encapsulated C-dots and implanted in mouse brain. In vivo CEST MRI was able to clearly differentiate labeled cells from non-labeled cells. The present findings may encourage new applications of C-dots for in vivo imaging in deep tissues, which is currently not possible using conventional fluorescent (near-infrared) C-dots.