Two receptor-like kinases required together for the establishment of Arabidopsis cotyledon primordia
Two receptor-like kinases required together for the establishment of Arabidopsis cotyledon primordia
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DOI：
10.1016/j.ydbio.2007.11.021
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发表时间：
2008-02-01
影响因子：
2.7
通讯作者：
Tax, Frans E.
中科院分区：
文献类型：
--
作者：
Nodine, Michael D.;Tax, Frans E.
区域间信号协调拟南芥胚胎的模式形成。然而，关于参与区域间通信的细胞和分子知之甚少。我们已经鉴定了两种相关的富含亮氨酸重复序列的受体样激酶（LRR-RLK），受体样蛋白激酶1（RPK 1）和TOADSTOOL 2（TOAD 2），它们是产生子叶原基的顶端胚胎结构域细胞类型的图案化所必需的。在突变体胚中，子叶原基和细胞类型的缺陷总是伴随着中央结构域原皮层图案化缺陷。此外，RPK 1-GFP和TOAD 2-GFP的翻译融合定位于中央域的原胚层细胞时，子叶原基是第一次可识别的。我们建议，RPK 1和TOAD 2主要需要维持中央域原胚层细胞的命运，这种关键的胚胎细胞类型的突变胚胎的损失导致图案缺陷，在其他地区的胚胎，包括未能启动子叶原基。(C)2007年爱思唯尔公司All rights reserved.
Inter-regional signaling coordinates pattern formation in Arabidopsis thaliana embryos. However, little is known regarding the cells and molecules involved in inter-regional communication. We have characterized two related leucine-rich repeat receptor-like kinases (LRR-RLKs), RECEPTOR-LIKE PROTEIN KINASE1 (RPK1) and TOADSTOOL2 (TOAD2), which are required together for patterning the apical embryonic domain cell types that generate cotyledon primordia. Central domain protoderm patterning defects were always observed subjacent to the defective cotyledon primordia, cell types in mutant embryos. In addition, RPK1-GFP and TOAD2-GFP translational fusions were both localized to the central domain protodermal cells when cotyledon primordia were first recognizable. We propose that RPK1 and TOAD2 are primarily required to maintain central domain protoderm cell fate and that the loss of this key embryonic cell type in mutant embryos results in patterning defects in other regions of the embryo including the failure to initiate cotyledon primordia. (C) 2007 Elsevier Inc. All rights reserved.