Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism
Enhancement of energy production by black ginger extract containing polymethoxy flavonoids in myocytes through improving glucose, lactic acid and lipid metabolism
复制标题
含有多甲氧基黄酮类化合物的黑姜提取物通过改善葡萄糖、乳酸和脂质代谢来增强肌细胞的能量产生
DOI：
--
复制
发表时间：
2016
影响因子：
3.3
通讯作者：
H. Shimoda
中科院分区：
文献类型：
--
作者：
K. Toda;Shogo Takeda;S. Hitoe;Seikou Nakamura;H. Matsuda;H. Shimoda
增强肌肉能量生产被认为可以改善运动功能，预防代谢综合征，包括糖尿病和糖尿病。黑姜（Kaempferia parviflora）在泰国被种植用于传统医学。最近的研究表明，黑姜提取物（KPE）激活棕色脂肪细胞，并在白色脂肪组织中进行脂解，这可能会治疗肥胖相关的脂质代谢功能障碍。然而，KPE对肌肉细胞中葡萄糖和脂质利用的影响尚未被研究。因此，我们评估了KPE及其组分对预分化（p）和分化（d）C2 C12成肌细胞中能量代谢的影响。KPE（0.1-10 μg/ml）加入pC 2C 12细胞分化过程中1周或处理dC 2C 12细胞24 h。培养后，评估葡萄糖和脂质代谢以及线粒体生物合成的参数。结果表明，KPE可增强两种细胞对2-脱氧葡萄糖和乳酸的摄取以及葡萄糖转运蛋白（GLUT）4和单羧酸转运蛋白（MCT）1的mRNA表达。pC 2C 12细胞过氧化物酶体增殖物激活受体γ共激活因子（PGC）-1α表达增强。此外，KPE增强ATP的产生和线粒体生物合成。KPE中5-羟基-7-甲氧基黄酮、5-羟基-3，7，4 ′-三甲氧基黄酮和5，7-二甲氧基黄酮等多甲氧基黄酮类化合物可促进GLUT 4和PGC-1α的表达。此外，KPE和5，7-二甲氧基黄酮还能促进5′ AMP活化蛋白激酶（AMPK）的磷酸化。总之，发现KPE及其多甲氧基黄酮增强心肌细胞的能量代谢。KPE可改善导致代谢综合征和运动功能障碍的肌肉代谢功能障碍。
Enhancement of muscular energy production is thought to improve locomotive functions and prevent metabolic syndromes including diabetes and lipidemia. Black ginger (Kaempferia parviflora) has been cultivated for traditional medicine in Thailand. Recent studies have shown that black ginger extract (KPE) activated brown adipocytes and lipolysis in white adipose tissue, which may cure obesity-related dysfunction of lipid metabolism. However, the effect of KPE on glucose and lipid utilization in muscle cells has not been examined yet. Hence, we evaluated the effect of KPE and its constituents on energy metabolism in pre-differentiated (p) and differentiated (d) C2C12 myoblasts. KPE (0.1–10 μg/ml) was added to pC2C12 cells in the differentiation process for a week or used to treat dC2C12 cells for 24 h. After culturing, parameters of glucose and lipid metabolism and mitochondrial biogenesis were assessed. In terms of the results, KPE enhanced the uptake of 2-deoxyglucose and lactic acid as well as the mRNA expression of glucose transporter (GLUT) 4 and monocarboxylate transporter (MCT) 1 in both types of cells. The expression of peroxisome proliferator-activated receptor γ coactivator (PGC)-1α was enhanced in pC2C12 cells. In addition, KPE enhanced the production of ATP and mitochondrial biogenesis. Polymethoxy flavonoids in KPE including 5-hydroxy-7-methoxyflavone, 5-hydroxy-3,7,4′-trimethoxyflavone and 5,7-dimethoxyflavone enhanced the expression of GLUT4 and PGC-1α. Moreover, KPE and 5,7-dimethoxyflavone enhanced the phosphorylation of 5′AMP-activated protein kinase (AMPK). In conclusion, KPE and its polymethoxy flavonoids were found to enhance energy metabolism in myocytes. KPE may improve the dysfunction of muscle metabolism that leads to metabolic syndrome and locomotive dysfunction.