The interaction between osteoclast-like cells and osteoblasts mediated by nanophase calcium phosphate-hybridized tendons
The interaction between osteoclast-like cells and osteoblasts mediated by nanophase calcium phosphate-hybridized tendons
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DOI：
10.1016/j.biomaterials.2004.03.039
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发表时间：
2005-03-01
期刊：
影响因子：
14
通讯作者：
Ochiai, N
中科院分区：
文献类型：
--
作者：
Mutsuzaki, H;Sakane, M;Ochiai, N
我们开发了一种利用交替浸泡过程将磷酸钙 (CaP) 与生物有机软组织杂交的新技术。通过这种技术，我们将 CaP 与移植的肌腱组织杂交，以与骨隧道结合。将肌腱交替浸泡在 Ca 和 NaHPO4 溶液中 10 分钟。长度为 30-50nm 的针状帽晶体（包括低结晶磷灰石）沉积在从表面到肌腱深处 200 针的胶原原纤维上和之间。光镜和透射电镜观察，术后1周植入肌腱上出现破骨细胞样细胞和成骨细胞，肌腱表面可见类骨质。术后 2 周，破骨细胞样细胞通过在表面形成 Howship 的腔隙样空间来解决肌腱问题，并且成骨细胞在这些空间中形成类骨质。观察到植入的肌腱和新形成的骨之间的直接粘合。术后3周，新形成的厚骨与肌腱表面牢固粘合。从这些结果中，我们得出结论，通过加速 CaP 杂交方法制备的肌腱有效增强破骨细胞样细胞和成骨细胞，将植入的肌腱与新形成的骨结合。 (C) 2004 Elsevier Ltd. 保留所有权利。
We developed a novel technique of hybridizing calcium phosphate (CaP) with bioorganic soft tissue using an alternating soaking process. By this technique, we hybridized CaP with a grafted tendon tissue to bond with a bone tunnel. Tendons were soaked in Ca and NaHPO4 solutions alternately for 10 min. Needle-like Cap crystals 30-50nm in length including low-crystalline apatite were deposited on and between collagen fibrils from the surface to 200 pin deep in the tendon. In light and transmission electron microscopic images, osteoclast-like cells and osteoblasts appeared on the implanted tendon and osteoid was observed on the tendon surface at I week postoperatively. At 2 weeks postoperatively, osteoclast-like cells resolved the tendon by forming Howship's lacuna-like spaces on the surfaces and osteoblasts formed osteoid in these spaces. Direct bonding between the implanted tendon and the newly formed bone was observed. At 3 weeks postoperatively, thick newly formed bone firmly bonded to tendon surface. From these results, we conclude that the tendons prepared by an accelerated CaP hybridization method efficiently enhance osteoclast-like cells and osteoblasts to bond the implanted tendons to newly formed bone. (C) 2004 Elsevier Ltd. All rights reserved.