Detection of cross-reacting murine I-J like determinants on a human subset of T8+ antigen binding, presenting and contrasuppressor cells.
Detection of cross-reacting murine I-J like determinants on a human subset of T8+ antigen binding, presenting and contrasuppressor cells.
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检测人类 T8 抗原结合、呈递和反抑制细胞子集上的交叉反应鼠 I-J 样决定簇。
DOI：
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发表时间：
1984
影响因子：
4.6
通讯作者：
J. Avery
中科院分区：
文献类型：
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作者：
T. Lehner;R. Brines;T. Jones;J. Avery
小鼠I-J基因产物存在于T抑制细胞（SC）和因子、巨噬细胞和反抑制细胞（CSC）中。然而，尚未报道鼠I-J的人类对应物。由于有强有力的证据表明，一些鼠抗Ia抗血清与人Ia抗原交叉反应，因此测试了小鼠抗I-J抗体可能与相应的人II类决定簇交叉反应的可能性。事实上，这揭示了测试的三种抗I-J单克隆抗体（MoAb）和两种抗血清与人单核细胞反应，并且与I-J抗体反应的T8+细胞或单核细胞（Mo）的比例显著大于T4+细胞或单核细胞。放射自显影证实了这一点，125 I-SA的抑制作用显著用SA结合和提呈T8+毛野豌豆贴壁细胞（VV-AC）与T4+辅助细胞的功能重建实验以及对SA特异性抗体形成细胞的评估表明，这种T8+辅助细胞的抗原提呈功能与SA结合和提呈T8+毛野豌豆贴壁细胞（VV-AC）的抗原提呈功能有关。用抗I-J和补体的单克隆抗体杀伤可显著抑制该亚群。此外，T8+ VV-AC的亚群也具有CSC的功能，因为抗I-J的MoAb和补体的杀伤作用显著抑制了反抑制功能。这与小鼠CSC中I-J基因产物的存在一致。然而，T8+ VV-NAC（非贴壁细胞）或单核细胞（Mo）的类似处理未能分别影响这些细胞的抑制或辅助功能。表型表征，抑制125 I-SA结合和重建实验的辅助和抑制功能，表明一个子集的T8+抗原结合，呈递和CSC可能表达的决定簇与鼠I-J分子交叉反应。
Murine I-J gene products have been found in T suppressor cells (SC) and factors, macrophages and contrasuppressor cells (CSC). However, a human counterpart of the murine I-J has not been reported. As there is strong evidence that some murine anti-Ia antisera cross-react with human Ia antigens, the possibility was tested that mouse anti-I-J antibodies might cross-react with corresponding human class II determinants. Indeed, this revealed that three anti-I-J monoclonal antibodies (MoAb) and two antisera tested react with human mononuclear cells and that a significantly greater proportion of T8+ than T4+ cells or monocytes (Mo) react with the I-J antibodies. This was corroborated by autoradiography with significant inhibition of 125I-SA (streptococcal antigen) binding to T8+ cells but not to Mo by the MoAb or antisera to murine I-J. Functional reconstitution experiments of T4+ helper cells with the SA binding and presenting T8+ Vicia villosa adherent cells (VV-AC) and assessment of specific antibody forming cells to SA suggest that the antigen presenting function of this T8+ subset can be significantly inhibited by killing with the MoAb to I-J and complement. Furthermore, the subset of T8+ VV-AC also functions as CSC, for killing with MoAb to I-J and complement significantly inhibited the contrasuppressor function. This is consistent with the presence of I-J gene products in murine CSC. However, similar treatment of T8+ VV-NAC (non-adherent cells) or monocytes (Mo) failed to affect the suppressor or accessory helper function of these cells, respectively. Phenotypic characterization, inhibition of 125I-SA binding and reconstitution experiments for helper and suppressor functions, suggest that a subset of T8+ antigen binding, presenting and CSC may express determinants cross-reacting with murine I-J molecules.