A Ratiometric Fluorescent Probe Based on ESIPT and AIE Processes for Alkaline Phosphatase Activity Assay and Visualization in Living Cells
A Ratiometric Fluorescent Probe Based on ESIPT and AIE Processes for Alkaline Phosphatase Activity Assay and Visualization in Living Cells
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DOI：
10.1021/am505150d
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发表时间：
2014-10-08
影响因子：
9.5
通讯作者：
Tang, Ben Zhong
中科院分区：
文献类型：
--
作者：
Song, Zhegang;Kwok, Ryan T. K.;Tang, Ben Zhong
碱性磷酸酶（ALP）活性被认为是医学诊断中的重要生物标志物。以磷酸化查尔酮衍生物为基础，研制了一种用于ALP活性测定和活细胞可视化的比率荧光探针。该探针可溶于水，在水性缓冲液中发出黄绿色。在碱性磷酸酶存在下，由于酶产物的形成和聚集，探针的发射逐渐变为深红色，并伴随比例荧光响应，其荧光既涉及激发态分子内质子转移过程，又涉及聚集诱导发射过程。线性比率荧光响应能够在0-150 mU/mL范围内体外定量ALP活性，检测限为0.15 mU/mL。该探针还显示出优异的生物相容性，这使其能够应用于活细胞中的ALP作图。
Alkaline phosphatase (ALP) activity is regarded as an important biomarker in medical diagnosis. A ratiometric fluorescent probe is developed based on a phosphorylated chalcone derivative for ALP activity assay and visualization in living cells. The probe is soluble in water and emits greenish-yellow in aqueous buffers. In the presence of ALP, the emission of probe changes to deep red gradually with ratiometric fluorescent response due to formation and aggregation of enzymatic product, whose fluorescence involves both excited-state intramolecular proton transfer and aggregation-induced emission processes. The linear ratiometric fluorescent response enables in vitro quantification of ALP activity in a range of 0-150 mU/mL with a detection limit of 0.15 mU/mL. The probe also shows excellent biocompatibility, which enables it to apply in ALP mapping in living cells.