Medium composition optimization, structural characterization, and antioxidant activity of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi
Medium composition optimization, structural characterization, and antioxidant activity of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi
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药用蘑菇灵芝胞外多糖的培养基组成优化、结构表征及抗氧化活性
DOI：
10.1016/j.ijbiomac.2018.11.274
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发表时间：
2019-03-01
影响因子：
8.2
通讯作者：
Dai, Yu-Cheng
中科院分区：
文献类型：
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作者：
Si, Jing;Meng, Ge;Dai, Yu-Cheng
为有效开发利用天然抗氧化剂，采用响应面法对灵芝胞外多糖（GLEPS）发酵培养基进行优化。通过Plackett-Burman设计、最陡爬坡路径和Box-Behnken设计，得到GLEPS生产的最佳培养基如下：葡萄糖（59.62 g/L），酵母提取物（10.03 g/L），CaCO3（0.2克/升），硫胺素（45.13 mg/L）、KH 2 PO 4（1.0 g/L）、蛋白胨（1.5 g/L）、吐温80（10.26 mL/L）、硫酸锌（0.3 g/L）、甘露醇（1.5 g/L）、MgSO 4（0.5 g/L）和天冬氨酸（8.86 g/L）。GLEPS产量为3.57 ± 0.21 g/L，是单独使用基础培养基的3.16倍。所得GLEPS富含糖醛酸、o-甘露糖、L-鼠李糖和o-葡萄糖，是具有高分子量（475，000 kDa和21.6kDa，87.97%）的杂多糖。结果表明，糖醛酸含量较高的GLEPS具有较强的体外抗氧化活性，其抗氧化活性主要通过清除自由基、还原能力和螯合过渡金属催化作用实现。结果表明，响应面法是预测GLEPS产量最大化所需培养基组成的有效工具，GLEPS具有较强的抗氧化活性，可作为一种新型的天然抗氧化剂应用于功能性食品或药物中。(C)2018 Elsevier B. V.版权所有。
To contribute towards effective exploitation and utilization of natural antioxidants, response surface methodology (RSM) was employed to optimize the medium composition for the production of exopolysaccharides from the medicinal mushroom Ganoderma lingzhi (GLEPS). An optimal medium for GLEPS production was gave through Plackett-Burman design, path of steepest ascent, and Box-Behnken design as follows: glucose (59.62 g/L), yeast extract (10.03 g/L), CaCO3 (0.2 g/L), thiamine (45.13 mg/L), KH2PO4 (1.0 g/L), peptone (1.5 g/L), Tween 80 (10.26 mL/L), ZnSO4 (0.3 g/L), mannitol (1.5 g/L), MgSO4 (0.5 g/L), and aspartate (8.86 g/L). The GLEPS yield obtained was 3.57 0.21 g/L-3.16-fold higher than that produced in basal medium alone. The resulting GLEPS rich in uronic acid, o-mannose, L-rhamnose, and o-glucose, was a heteropolysaccharide with high-molecular weights (475,000 kDa and 21.6 kDa, 87.97%). It was demonstrated that the GLEPS with higher carbohydrate and uronic acid contents exhibited strong in vitro antioxidant activities via radical scavenging, reductive capacity, and chelation of transition metal catalysis. These findings indicated that RSM is an efficient tool to predict the composition of culture medium required for maximizing GLEPS yield, and GLEPS had potent antioxidant activities and could be explored as a novel natural antioxidant in functional food or medicine. (C) 2018 Elsevier B.V. All rights reserved.