Biocatalyst development by directed evolution.
Biocatalyst development by directed evolution.
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DOI：
10.1016/j.biortech.2012.01.054
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发表时间：
2012-07
影响因子：
11.4
通讯作者：
Zhao H
中科院分区：
文献类型：
--
作者：
Wang M;Si T;Zhao H
生物催化已经成为传统化学工艺的重要补充，用于生产大宗化学品和药物。为了克服天然存在的酶的局限性，定向进化已成为改善生物催化剂的关键特性，如热稳定性，活性，选择性和对有机溶剂的耐受性，用于工业应用的最重要的工具。突变体库创建和高通量筛选的最新进展极大地促进了新型和改进的生物催化剂的工程化。这篇综述提供了一个更新的最新发展，在使用定向进化工程生物催化剂的实际应用。
Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly facilitated the engineering of novel and improved biocatalysts. This review provides an update of the recent developments in the use of directed evolution to engineer biocatalysts for practical applications.