Gold-nanorod-based sensing of sequence specific HIV-1 virus DNA by using hyper-Rayleigh scattering spectroscopy
Gold-nanorod-based sensing of sequence specific HIV-1 virus DNA by using hyper-Rayleigh scattering spectroscopy
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DOI：
10.1002/chem.200701850
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发表时间：
2008-01-01
影响因子：
4.3
通讯作者：
Ray, Paresh Chandra
中科院分区：
文献类型：
--
作者：
Darbha, Gopala Krishna;Rai, Uma Shanker;Ray, Paresh Chandra
由人类免疫缺陷病毒（HIV）引起的传染病仍然是全世界人类的主要杀手，并破坏非洲、亚洲和中东社会的稳定。由于需要检测HIV病毒序列的存在，在这里，我们证明了金纳米棒的二阶非线性光学（NLO）性质可以用于筛选HIV-1病毒DNA序列，无需任何修饰，具有良好的灵敏度（100皮摩尔）和选择性（单碱基对错配）。当无标记的145-mer ss-gag基因DNA与100 pM靶DNA杂交时，超瑞利散射（HRS）强度增加45倍。HRS强度变化的机制进行了讨论与实验证据更高的多极贡献的非线性光学响应的金纳米棒。
Infectious diseases caused by the human immunodeficiency virus (HIV) remain the leading killers of human beings worldwide, and function to destabilize societies in Africa, Asia, and the Middle East. Driven by the need to detect the presence of HIV viral sequence, here we demonstrate that the second-order nonlinear optical (NLO) properties of gold nanorods can be used for screening HIV-1 viral DNA sequence without any modification, with good sensitivity (100 picomolar) and selectivity (single base-pair mismatch). The hyper-Rayleigh scattering (HRS) intensity increases 45 times when a label-free 145-mer, ss-gag gene DNA, was hybridized with 100 pM target DNA. The mechanism of HRS intensity change has been discussed with experimental evidence for higher multipolar contribution to the NLO response of gold nanorods.