Intraflagellar transport protein 27 is a small G protein involved in cell-cycle control
Intraflagellar transport protein 27 is a small G protein involved in cell-cycle control
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DOI：
10.1016/j.cub.2006.12.040
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发表时间：
2007-02-06
期刊：
影响因子：
9.2
通讯作者：
Rosenbaum, Joel
中科院分区：
文献类型：
--
作者：
Qin, Hongmin;Wang, Zhaohui;Rosenbaum, Joel
背景资料：鞭毛内转运（IFT）是纤毛/鞭毛（可互换的术语）膜和运动和感觉纤毛的微管轴丝之间的运动过程。由复合物A和B组成的多多肽IFT颗粒将鞭毛前体携带到由驱动蛋白驱动的鞭毛尖端处的组装位点（顺行），并将由细胞质动力蛋白驱动的产物从尖端翻转回到细胞质（逆行）。IFT是必不可少的组装和维护几乎所有的真核生物纤毛和鞭毛，和突变影响无论是IFT马达或IFT颗粒多肽导致无法组装正常的鞭毛或在cilia.Results的感觉功能的缺陷：我们发现，IFT复合物B多肽，IFT 27，是一个Rab-like小G蛋白。通过RNA干扰降低IFT 27的水平降低了其他复合物A和B蛋白的水平，表明该蛋白有助于维持两种IFT复合物的稳定性。此外，除了其在鞭毛组装中的作用之外，IFT多肽中的IFT 27是独特的，因为其部分敲低导致胞质分裂和细胞周期延长中的缺陷，并且更完整的敲低是lethal.Conclusion：IFT 27，一种小G蛋白，是越来越多的鞭毛蛋白之一，现在已知其在细胞周期控制中具有作用。
Background: Intraflagellar transport (IFT) is a motility process operating between the ciliary/flagellar (interchangeable terms) membrane and the microtubular axoneme of motile and sensory cilia. Multi polypeptide IFT particles, composed of complexes A and B, carry flagellar precursors to their assembly site at the flagellar tip (anterograde) powered by kinesin, and turnover products from the tip back to the cytoplasm (retrograde) driven by cytoplasmic dynein. I FT is essential for the assembly and maintenance of almost all eukaryotic cilia and flagella, and mutations affecting either the IFT motors or the IFT particle polypeptides result in the inability to assemble normal flagella or in defects in the sensory functions of cilia.Results: We found that the IFT complex B polypeptide, IFT27, is a Rab-like small G protein. Reduction of the level of IFT27 by RNA interference reduces the levels of other complex A and B proteins, suggesting that this protein is instrumental in maintaining the stability of both IFT complexes. Furthermore, in addition to its role in flagellar assembly, IFT27 is unique among IFT polypeptides in that its partial knockdown results in defects in cytokinesis and elongation of the cell cycle and a more complete knockdown is lethal.Conclusion: IFT27, a small G protein, is one of a growing number of flagellar proteins that are now known to have a role in cell-cycle control.