Guar gum-derived galactomannan induces inflammatory responses and increased energy expenditure in the intestine
Guar gum-derived galactomannan induces inflammatory responses and increased energy expenditure in the intestine
复制标题
瓜尔胶衍生的半乳甘露聚糖可诱导炎症反应并增加肠道能量消耗
DOI：
10.1039/d1fo01143j
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发表时间：
2021-06-02
期刊：
影响因子：
6.1
通讯作者：
Zuo，Jianjun
中科院分区：
文献类型：
--
作者：
Wang，Weiwei;Fu，Rao;Zuo，Jianjun
瓜尔胶衍生的半乳甘露聚糖（GGGM）在食品工业中的应用由来已久，但其不良影响却鲜有报道。半乳甘露聚糖被认为具有与某些病原体的表面组分相似的结构，因此进行本研究以研究口服GGGM是否会引起假设与肠道炎症反应相关的生理效应。结果表明，口服GGGM会导致小鼠生长性能下降，脾脏和附睾脂肪相对重量增加，血清和肝脏中α1-酸性糖蛋白含量升高。关于能量代谢相关的指标，肠乳酸脱氢酶和琥珀酸脱氢酶的活性在体内和体外实验中均通过GGGM处理而增加，后者还显示肠上皮细胞沿着这些细胞响应于GGGM处理的活力降低而消耗还原糖的升高。值得注意的是，GGGM治疗引发了肠道炎症反应，这通过体内和体外肠道炎性细胞因子如TNF-α和IL-6的表达增加来证明，这至少部分地导致了肠道能量消耗增加和生长迟缓。这项研究的结果可以扩大我们对GGGM管理的了解，并为含GGGM食品的消费提供综合见解。
Guar gum-derived galactomannan (GGGM) has been widely used in the food industry for a long time and its adverse impacts have been scarcely reported. Galactomannan is considered to have a structure similar to the surface components of certain pathogens, and the present study was thus conducted to investigate if oral administration of GGGM could cause physiological effects that were hypothesized to be related to intestinal inflammatory responses. The results showed that oral administration of GGGM resulted in compromises on growth performance, an increase of the relative weight of spleen and epididymal fat, and an elevation of the α1-acid glycoprotein content in both serum and livers of mice. With regard to energy metabolism-related indices, the activities of intestinal lactic dehydrogenase and succinic dehydrogenase were all increased by the GGGM treatment in both in vivo and in vitro experiments, the latter of which also showed an elevation in the consumption of reducing sugar by intestinal epithelial cells along with a reduced viability of these cells in response to the GGGM treatment. Notably, the GGGM treatment triggered intestinal inflammatory responses that were evidenced by the increased expression of intestinal inflammatory cytokines such as TNF-α and IL-6 both in vivo and in vitro, which were at least partially responsible for the increased energy expenditure in the intestine and the retardation of growth. The results of this study could expand our knowledge of GGGM administration and provide integrated insights into the consumption of GGGM-containing foods.