Global Gene Deletion Analysis Exploring Yeast Filamentous Growth
Global Gene Deletion Analysis Exploring Yeast Filamentous Growth
复制标题
DOI：
10.1126/science.1224339
复制
发表时间：
2012-09-14
期刊：
影响因子：
56.9
通讯作者：
Boone, Charles
中科院分区：
文献类型：
--
作者：
Ryan, Owen;Shapiro, Rebecca S.;Boone, Charles
从单细胞芽殖酵母到丝状形式的双态转换使酿酒酵母能够觅食营养物质，机会致病菌白色念珠菌能够侵入人体组织并逃避免疫系统。我们构建了一组全基因组的靶向缺失等位基因，并将其导入丝状S。酿酒酵母菌株，Sigma 1278b。我们确定了参与形态学上不同形式的菌丝形成的基因：单倍体侵入性生长、生物膜形成和二倍体假菌丝生长。独特的基因似乎是每个程序的基础，但我们也发现了在丝状生长中具有一般作用的核心基因，包括MFG1（YDL233w），其产物结合两种形态发生转录因子Flo8和Mss11，并在两种S.酿酒酵母和C.白色念珠菌。
The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, Sigma 1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.