The distribution of transient receptor potential melastatin-8in the rat soft palate, epiglottis, and pharynx.
The distribution of transient receptor potential melastatin-8in the rat soft palate, epiglottis, and pharynx.
复制标题
瞬时受体电位melastatin-8在大鼠软腭、会厌和咽部的分布。
DOI：
10.1007/s10571-012-9888-1
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发表时间：
2013
影响因子：
4
通讯作者：
Ichikawa H.
中科院分区：
文献类型：
--
作者：
Sato T;Fujita M;Kano M;Hosokawa H;Kondo T;Suzuki T;Kasahara E;Shoji N;Sasano T;Ichikawa H.
在大鼠软腭、会厌和咽部进行瞬时受体电位melastatin-8（TRPM 8）（冷和薄荷醇受体）的免疫组织化学。TRPM 8免疫反应（IR）神经纤维位于粘膜上皮下方，偶尔穿透上皮。这些神经纤维在软腭后部和咽部的鼻口和喉部的边缘区域丰富。会厌没有这种神经纤维。免疫荧光双标法显示，咽部和软腭部TRPM 8-IR神经纤维大多不表达降钙素基因相关肽（CGRP-IR）。逆行追踪法还证实，支配咽部的颈静脉和岩神经节中分别有30.1%和8.7%的感觉神经元含有TRPM 8-IR。在这些神经元中，TRPM 8和CGRP-IR的共表达非常罕见。在结状神经节中，咽部神经元缺乏TRPM 8-IR，软腭和咽部的味蕾样结构中含有4-9个TRPM 8-IR细胞。在会厌中，喉侧的粘膜上皮具有大量TRPM 8-IR细胞。目前的研究表明，TRPM 8可以响应冷刺激时，食物和饮料通过口腔和咽腔。
Immunohistochemistry for transient receptor potential melastatin-8 (TRPM8), the cold and menthol receptor, was performed on the rat soft palate, epiglottis and pharynx. TRPM8-immunoreactive (IR) nerve fibers were located beneath the mucous epithelium, and occasionally penetrated the epithelium. These nerve fibers were abundant in the posterior portion of the soft palate and at the border region of naso-oral and laryngeal parts of the pharynx. The epiglottis was free from such nerve fibers. The double immunofluorescence method demonstrated that TRPM8-IR nerve fibers in the pharynx and soft palate were mostly devoid of calcitonin gene-related peptide-immunoreactivity (CGRP-IR). The retrograde tracing method also demonstrated that 30.1 and 8.7 % of sensory neurons in the jugular and petrosal ganglia innervating the pharynx contained TRPM8-IR, respectively. Among these neurons, the co-expression of TRPM8 and CGRP-IR was very rare. In the nodose ganglion, however, pharyngeal neurons were devoid of TRPM8-IR. Taste bud-like structures in the soft palate and pharynx contained 4–9 TRPM8-IR cells. In the epiglottis, the mucous epithelium on the laryngeal side had numerous TRPM8-IR cells. The present study suggests that TRPM8 can respond to cold stimulation when food and drinks pass through oral and pharyngeal cavities.