Rapid Airborne Influenza Virus Quantification Using an Antibody-Based Electrochemical Paper Sensor and Electrostatic Particle Concentrator
Rapid Airborne Influenza Virus Quantification Using an Antibody-Based Electrochemical Paper Sensor and Electrostatic Particle Concentrator
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DOI：
10.1021/acs.est.0c00441
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发表时间：
2020-09-01
影响因子：
11.4
通讯作者：
Jang, Jaesung
中科院分区：
文献类型：
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作者：
Bhardwaj, Jyoti;Kim, Myeong-Woo;Jang, Jaesung
空气传播的流感病毒是导致严重呼吸道疾病的罪魁祸首，大多数空气传播病毒的检测方法都是基于提取核酸。为此，研制了一种基于垂直流动分析的电化学纸免疫传感器，用于便携式静电粒子浓缩器(EPC)采样后对空气中H1N1流感病毒的快速定量。研究了抗流感核蛋白抗体(NP-Abs)和抗流感血凝素抗体(HA-Abs)在纸质传感器上的作用以及带电晕和不带电晕的非脉冲高压静电场对病毒检测的影响。表面(HA)蛋白的抗原性丧失是由H_2O_2通过脂质氧化产生的自由基和O-1(2)在高压电场作用下通过直接的蛋白质过氧化造成的。然而，流感病毒NP的抗原性损失很小，使用基于NP-Ab的纸传感器采集60min后，EPC中H1N1病毒的浓度是生物采样器的160多倍。这种带有EPC的基于NP-Ab的纸质传感器提供了与定量聚合酶链式反应(QPCR)相当的测量结果，但速度快得多，在其他空气微生物和珠子存在的情况下，对H1N1流感病毒具有特异性，并且比酶联免疫吸附试验和qPCR更具成本效益。
Airborne influenza viruses are responsible for serious respiratory diseases, and most detection methods for airborne viruses are based on extraction of nucleic acids. Herein, vertical-flow-assay-based electrochemical paper immunosensors were fabricated to rapidly quantify the influenza H1N1 viruses in air after sampling with a portable electrostatic particle concentrator (EPC). The effects of antibodies, anti- influenza nucleoprotein antibodies (NP-Abs) and anti-influenza hemagglutinin antibodies (HA-Abs), on the paper sensors as well as nonpulsed high electrostatic fields with and without corona charging on the virus measurement were investigated. The antigenicity losses of the surface (HA) proteins were caused by H2O2 via lipid oxidation-derived radicals and O-1(2) via direct protein peroxidation upon exposure of a high electrostatic field. However, minimal losses in antigenicity of NP of the influenza viruses were observed, and the concentration of the H1N1 viruses was more than 160 times higher in the EPC than the BioSampler upon using NP-Ab based paper sensors after 60 min collection. This NP-Ab-based paper sensors with the EPC provided measurements comparable to quantitative polymerase chain reaction (qPCR) but much quicker, specific to the influenza H1N1 viruses in the presence of other airborne microorganisms and beads, and more cost-effective than enzyme-linked immunosorbent assay and qPCR.