An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
An improved RT-IPCR for detection of pyrene and related polycyclic aromatic hydrocarbons.
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DOI：
10.1016/j.bios.2015.11.037
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发表时间：
2016-04
影响因子：
12.6
通讯作者：
X. Meng;Y. S. Li;Y. Zhou;Y. Sun;B. Qiao;C. Si;P. Hu;S. Y. Lu;H. Ren;Z. Liu;H. Qiu;J. Liu
中科院分区：
文献类型：
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作者：
X. Meng;Y. S. Li;Y. Zhou;Y. Sun;B. Qiao;C. Si;P. Hu;S. Y. Lu;H. Ren;Z. Liu;H. Qiu;J. Liu
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous homogeneous chemicals which are well known by carcinogens, mutagens and endocrine disorder. Here, an improved real-time immuno-PCR (RT-IPCR) was developed for detection of pyrene and its homologs in water samples. The PAHs in sample compete with pyrene-modified DNA to bind with monoclonal antibody (McAb) coated on PCR plate. The reporter DNA was exponentially amplified by real-time PCR instrument using Fast Start universal SYBR Green Master (ROX) kit. Only two reaction steps were needed to accomplish the detection. The assay had a good linear range from 5 pmol L−1to 5 nmol L−1with a detection limit of 3.5 pmol L−1. For application assay, the average recoveries from tap water, lake water and mineral water were 98.4%, 98.2% and 99.7%, respectively which showed a good correlation (R2=0.9906) with those from GC–MS. The results indicated that the improved RT-IPCR seems to be a potential method for simple and ultrasensitive detection of pyrene and some homologues in environment water samples.
