Rhodoplanes piscinae sp nov isolated from pond water
Rhodoplanes piscinae sp nov isolated from pond water
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DOI：
10.1099/ijs.0.037663-0
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发表时间：
2012-12-01
影响因子：
2.8
通讯作者：
Ramana, Ch. V.
中科院分区：
文献类型：
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作者：
Chakravarthy, S. Kalyana;Ramaprasad, E. V. V.;Ramana, Ch. V.
从淡水鱼塘和工业废水中分离到革兰氏阴性杆状光养紫色无硫细菌JA266(T)和JA333两株。两株菌株均能进行光养和化养生长。螺旋藻黄质系列中的细菌叶绿素a和类胡萝卜素作为光合色素存在。两菌株的主要脂肪酸均为C-18:1 ω - 7c(>65%)，同时存在少量的11-甲基CC18:1 ω - 7c、C-16:0、C-16:1 ω - 7c和C-18:0。这两种菌株都具有片层型的胞内光合膜。醌类主要成分为泛醌-10 （Q(10)）和红醌-10 （RQ(10)）。二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱是主要的极性脂质，而少量的氨基脂质（AL1、AL2）和一种未识别的脂质（L1）在两种菌株中都是共同的。菌株JA266(T)和JA333的DNA G+C含量分别为71.3%和69.9%。基于16S rRNA基因序列的系统发育分析表明，两株菌株均属于Alphaproteobacteria纲Rhodoplanes属成员。菌株JA266(T)和JA333与serdoplane serenus TUT3530(T)的基因序列相似性分别为98.7和98.9%，与elegans AS130(T)的基因序列相似性分别为96.4和96.5%，与其他rhodoplane属成员的基因序列相似性小于96%，165 rRNA基因序列相似性为99.3%，DNA-DNA杂交结果表明，两株菌株具有较高的亲缘性（84.7%）。此外，这两株菌株的DNA与小雪鼠型菌株TUT3530(T)的DNA亲缘性均小于65%。根据表型和基因型数据，建议将菌株JA266(T)归类为Rhodoplanes属的新种，种名为Rhodoplanes piscinae sp. nov.，该新种的型菌株为JA266(T) (=JCM 14934(T)=KCTC 5627(T))，菌株JA333 （=NBRC 107574=KCTC 5962）为附加菌株。
Two strains (JA266(T) and JA333) of Gram-negative, rod-shaped, phototrophic, purple non-sulfur bacteria were isolated from a freshwater fish pond and an industrial effluent. Both strains were capable of phototrophic and chemotrophic growth. Bacteriochlorophyll a and carotenoids of the spirilloxanthin series were present as photosynthetic pigments. The major fatty acid for both strains was C-18:1 omega 7c (>65%), with minor amounts of 11-methyl CC18:1 omega 7C, C-16:0, C-16:1 omega 7c and C-18:0 also present. Both strains have the lamellar type of intracellular photosynthetic membranes. Ubiquinone-10 (Q(10)) and rhodoquinone-10 (RQ(10)) were present as primary quinone components. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine were the major polar lipids, while minor amounts of amino lipids (AL1, AL2) and an unidentified lipid (L1) were common to both strains. The DNA G+C contents of strains JA266(T) and JA333 were 71.3 and 69.9 mol%, respectively. Phylogenetic analysis on the basis of 16S rRNA gene sequences showed that both strains clustered with members of the genus Rhodoplanes in the class Alphaproteobacteria. Strains JA266(T) and JA333 had gene sequence similarity of 98.7 and 98.9% with Rhodoplanes serenus TUT3530(T), 96.4 and 96.5% with Rhodoplanes elegans AS130(T), respectively, and less than 96% with other members of the genus Rhodoplanes. 165 rRNA gene sequence similarity between the two strains was 99.3% and they exhibited high (84.7%) relatedness based on DNA-DNA hybridization. Furthermore, both strains had less than 65% DNA DNA relatedness with the type strain R. serenus TUT3530(T). On the basis of phenotypic and genotypic data, it is proposed that strain JA266(T) be classified as a novel species of the genus Rhodoplanes, with the species name Rhodoplanes piscinae sp. nov. The type strain of the proposed novel species is JA266(T) (=JCM 14934(T)=KCTC 5627(T)), while strain JA333 (=NBRC 107574=KCTC 5962) is an additional strain.