Integrated proteomics, metabolomics and physiological analyses for dissecting the toxic effects of halosulfuron-methyl on soybean seedlings (Glycine max merr.)
Integrated proteomics, metabolomics and physiological analyses for dissecting the toxic effects of halosulfuron-methyl on soybean seedlings (Glycine max merr.)
复制标题
综合蛋白质组学、代谢组学和生理分析，剖析甲基卤磺隆对大豆幼苗的毒性作用 (Glycine max merr.)
DOI：
10.1016/j.plaphy.2020.10.033
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发表时间：
2020-12-01
影响因子：
6.5
通讯作者：
Tan, Huihua
中科院分区：
文献类型：
--
作者：
Li, Yuanfu;Zhang, Qiannan;Tan, Huihua
氟磺隆(HSM)是一种除草剂，广泛用于作物生产中的莎草和阔叶杂草的防除，但其环境残留会对非靶标作物造成危害。本论文利用蛋白质组学和代谢组学的方法，探讨了HSM对大豆的药害机理。大豆幼苗经0.01、0.05和0.5 mg/L的HSM处理8d后，HSM处理显著降低了幼苗的叶绿素和类胡萝卜素含量。此外，叶绿素a的荧光受到严重影响。高温胁迫下幼苗体内谷胱甘肽、过氧化氢和丙二醛含量以及抗氧化酶活性均显著增加。此外，与对照相比，HSM处理的幼苗中参与三羧酸(TCA)循环的5种酶--α-酮戊二酸脱氢酶、异柠檬酸脱氢酶、乌头酸酶、苹果酸脱氢酶和琥珀酸脱氢酶受到不同程度的抑制。蛋白质组学结果表明，多种差异丰富的蛋白质参与了叶绿素的合成，光系统过程和叶绿体ATP合成酶表达下调。代谢组学分析表明，参与TCA循环的代谢物显著减少。此外，与活性氧解毒相关的代谢物和蛋白质也积累了。综上所述，HSM对大豆的药害机制主要是通过破坏光合作用机制、抑制叶绿素合成、干扰TCA循环和引起氧化胁迫来实现的。这些结果为研究磺酰基脲类除草剂对非靶标作物的毒性机理提供了新的思路。
Halosulfuron methyl (HSM) is a herbicide widely used to control sedge and broad-leaved weeds during crop production, but its environmental residue may damage non-target crops. Here, proteomics and metabolomics methods were used to explore the phytotoxicity mechanisms of HSM against soybean (Glycine max Merr.). Soybean seedlings were exposed to 0.01, 0.05 and 0.5 mg/L HSM for 8 d. The HSM applications significantly reduced chlorophyll and carotenoid contents in HSM-treated seedlings. Additionally, chlorophyll a fluorescence was seriously affected. The glutathione, hydrogen peroxide and malondialdehyde contents, as well as antioxidant enzyme activities, significantly increased in seedlings exposed to HSM. Furthermore, five enzymes involved in the tricarboxylic acid (TCA) cycle, alpha-ketoglutarate dehydrogenase, isocitrate dehydrogenase, aconitase, malic dehydrogenase and succinate dehydrogenase, were inhibited to varying degrees in HSM-treated seedlings compared with controls. Proteomics results showed multiple differentially abundant proteins involved in chlorophyll synthesis, photosystem processes and chloroplast ATP synthetase were down-regulated. Metabolomics analyses revealed that metabolites involved in the TCA cycle decreased significantly. Moreover, metabolites and proteins related to reactive oxygen species detoxification accumulated. In conclusion, the phytotoxicity mechanisms of HSM against soybean mainly act by damaging the photosynthetic machinery, inhibiting chlorophyll synthesis, interrupting the TCA cycle and causing oxidative stress. These results provide new insights into the toxicity mechanisms of sulfonylurea herbicides against non-target crops.