Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy.
Measurement and correction of in vivo sample aberrations employing a nonlinear guide-star in two-photon excited fluorescence microscopy.
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DOI：
10.1364/boe.2.003135
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发表时间：
2011-11-01
影响因子：
3.4
通讯作者：
Loza-Alvarez P
中科院分区：
文献类型：
--
作者：
Aviles-Espinosa R;Andilla J;Porcar-Guezenec R;Olarte OE;Nieto M;Levecq X;Artigas D;Loza-Alvarez P
我们证明，样品引起的像差可以在非线性显微镜测量。这利用了双光子激发荧光在样品内部自然产生局部点源的事实：非线性导星（NL-GS）。然后可以使用Shack-Hartmann传感器记录从NL-GS发射的波前。记录的样品像差的补偿由可变形反射镜在单个步骤中执行。该技术应用于固定的和体内的生物样品，在某些情况下，显示出在总收集的信号强度上的一个数量级以上的改善。
We demonstrate that sample induced aberrations can be measured in a nonlinear microscope. This uses the fact that two-photon excited fluorescence naturally produces a localized point source inside the sample: the nonlinear guide-star (NL-GS). The wavefront emitted from the NL-GS can then be recorded using a Shack-Hartmann sensor. Compensation of the recorded sample aberrations is performed by the deformable mirror in a single-step. This technique is applied to fixed and in vivo biological samples, showing, in some cases, more than one order of magnitude improvement in the total collected signal intensity.