Fast STED microscopy with continuous wave fiber lasers
Fast STED microscopy with continuous wave fiber lasers
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DOI：
10.1364/oe.18.001302
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发表时间：
2010-01-18
期刊：
影响因子：
3.8
通讯作者：
Hell, Stefan W.
中科院分区：
文献类型：
--
作者：
Moneron, Gael;Medda, Rebecca;Hell, Stefan W.
我们报告了可见光范围内的快速光束扫描受激发射损耗 (STED) 显微镜，用于提高分辨率，紧凑、低成本、交钥匙连续波 (CW) 光纤激光器，发射波长为 592 nm。各种样品的焦平面空间分辨率为 35 至 65 nm，包括荧光纳米颗粒、使用非详尽选择的 5 种常用有机荧光标记物进行免疫染色的细胞，以及表达黄色荧光蛋白 Citrine 的活细胞。活细胞内质网 (ER) 的视频展示了 CW-STED 和快速光束扫描直接组合的潜力，该视频由 100 个帧 (6 μm x 12 μm) 组成，每个帧的采集时间都不到 0.2 秒。 (C) 2010 美国光学学会
We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm in the focal plane are shown for various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection of 5 commonly used organic fluorescent markers, and living cells expressing the yellow fluorescent protein Citrine. The potential of the straightforward combination of CW-STED and fast beam scanning is illustrated in a movie of the endoplasmic reticulum (ER) of a living cell, composed of 100 frames (6 mu m x 12 mu m), each of them acquired in a time shorter than 0.2 s. (C) 2010 Optical Society of America