DIFFERENTIAL EXPRESSION OF ANGIOTENSIN RECEPTOR 1A AND 1B IN MOUSE
DIFFERENTIAL EXPRESSION OF ANGIOTENSIN RECEPTOR 1A AND 1B IN MOUSE
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DOI：
10.1152/ajpendo.1994.267.2.e260
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发表时间：
1994-08-01
影响因子：
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通讯作者：
SIGMUND, CD
中科院分区：
文献类型：
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作者：
BURSON, JM;AGUILERA, G;SIGMUND, CD
在啮齿动物中，至少有两个不同的基因(AT(1A)和AT(1B))编码1型血管紧张素II(AT(1))受体。受体结合和Northern印迹分析已清楚地证明在许多组织中存在AT(1)受体和AT(1)受体mRNA，但无法区分表达哪种1型受体亚型。建立逆转录-聚合酶链式反应-限制性片段长度多态性(RT-PCR-RFLP)分析方法，区分不同亚型表达的mRNA。在成年小鼠的肾脏、肝脏、肾上腺、卵巢、脑、睾丸、脂肪组织、肺和心脏中，AT(1A)的表达明显。AT(1B)在这些组织中大部分缺失，但在脑、睾丸和肾上腺中可检测到。16.5天或18.5天的胎儿肾脏、肝脏、脑、肺或心脏中的表达没有明显差异，只有胎盘中有明显的AT(1A)表达。使用一组仅特异扩增AT(1B)mRNA的引物，证实了AT(1B)在肾上腺、脑和睾丸中的表达。在肾素表达的小鼠肾肿瘤细胞株As4.1中也检测到AT(1A)和AT(1B)的表达。使用AT(1)和AT(2)受体拮抗剂进行的受体结合和竞争分析表明，细胞表面只有AT(1)受体存在。Northern印迹检测到极低水平的AT(1)受体mRNA，RT-PCR-RFLP分析表明该细胞仅表达AT(1A)亚型。尽管AT(1A)和AT(1B)基因的编码序列有很高的同源性，但它们表现出不同的组织特异性表达谱。
At least two distinct genes (AT(1A) and AT(1B)) encode type 1 angiotensin II (AT(1)) receptors in rodents. Receptor binding and Northern blot analysis have clearly demonstrated the presence of AT(1) receptors and AT(1)-receptor mRNA in many tissues but fail to differentiate which type 1 receptor subtype is expressed. A reverse-transcriptase polymerase chain reaction restriction fragment length polymorphism (RT-PCR-RFLP) assay was developed to differentiate the expressed mRNA by subtype. Expression of AT(1A) was clearly evident in kidney, liver, adrenal gland, ovary, brain, testes, adipose tissue, lung, and heart of adult mice. AT(1B) was absent from most of these tissues but was detectable in brain, testes, and adrenal gland. No significant differences in expression were evident in kidney, liver, brain, lung, or heart from 16.5- or 18.5-gestation-day fetuses, and only AT(1A) was evident in placenta. Expression of AT(1B) was confirmed in adrenal gland, brain, and testes, using a primer set that specifically amplifies only AT(1B) mRNA. Expression of AT(1A) and AT(1B) was also examined in As4.1 cells, a renin-expressing mouse kidney tumoral cell line. Receptor binding and competition assays using AT(1)- and AT(2)-receptor antagonists revealed that only AT(1) receptors are present on the cell surface. Extremely low levels of AT(1)-receptor mRNA was detected by Northern blot, and RT-PCR-RFLP analysis revealed that only the AT(1A) subtype is expressed in this cell line. Despite the high homology between the coding sequence of the AT(1A) and AT(1B) genes, they exhibit disparate tissue-specific expression profiles.