Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus.
Association between systemic leptin and neurotensin concentration in adult individuals with and without type 2 diabetes mellitus.
复制标题
DOI：
10.1007/s40618-018-0845-9
复制
发表时间：
2018-10-01
影响因子：
5.4
通讯作者：
Cavallo, M G
中科院分区：
文献类型：
--
作者：
Barchetta, I;Ciccarelli, G;Cavallo, M G
目得：瘦素（Leptin）是一种脂肪细胞因子，通过神经降压素（neurotensin，NT）部分介导的机制调节食欲和能量平衡。除了作为一种神经递质，NT在人体肠道中表达，它促进脂肪吸收，其循环水平与肥胖、2型糖尿病（T2 DM）和心血管疾病有关。循环瘦素和NT水平之间是否存在关系尚未研究。本研究的目的是检验血浆瘦素和NT浓度之间的联系在成人T2 DM或无T2 DM的假设。方法：我们招募了72名受试者（男/女：39/33;年龄：49.5± 10.6岁; BMI：26.5± 4.7 kg/m2），包括罗马大学糖尿病门诊的T2 DM患者（n=32）和健康对照。研究参与者进行代谢特征;血浆瘦素测定MILLIPLEX，Luminex，和原神经降压素（proNT），一个稳定的前体NT，通过荧光光度法夹心immunoassociation.Results：循环中位（25°-75°）瘦素水平为2.75（1.27-4.93）ng/mL，并没有T2 DM和非糖尿病受试者之间的差异。瘦素浓度与proNT直接相关（r=0.41; p=0.015）;更高的瘦素水平也与年龄、男性性别、肥胖、更高的HOMA-IR、收缩压和C反应蛋白相关。属于最高的前NT四分位数与更高的瘦素水平相关，与年龄，性别和其他混杂因素无关结论：循环瘦素与高proNT水平相关，与糖尿病、肥胖和代谢综合征无关;除了其对中枢瘦素信号传导的作用外，NT可能通过调节循环瘦素浓度影响能量平衡，可能是通过涉及肠道脂肪吸收的机制。
PURPOSE: Leptin is an adipokine which regulates appetite and energy balance through a mechanism partially mediated by neurotensin (NT) in central nervous system. Besides acting as a neurotransmitter, NT is expressed in human intestine where it promotes fat absorption and its circulating levels associate with obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease. Whether a relation exists between circulating leptin and NT levels has not been investigated yet. The aim of this study was to test the hypothesis of an association between plasma leptin and NT concentration in adults with or without T2DM.METHODS: We recruited a population of 72 subjects (M/F: 39/33; age: 49.5±10.6years; BMI: 26.5±4.7kg/m2) including individuals with T2DM (n=32) referring to our Diabetes Outpatient Clinics, Sapienza University of Rome, and healthy controls. Study participants underwent metabolic characterization; plasma leptin was measured by MILLIPLEX, Luminex, and proneurotensin (proNT), a stable precursor of NT, by chemiluminometric sandwich immunoassay.RESULTS: Circulating median (25°-75°) leptin levels were 2.75 (1.27-4.93)ng/mL and did not differ between T2DM and non-diabetic subjects. Leptin concentration directly correlated with proNT (r=0.41; p=0.015); higher leptin levels were also associated with age, male gender, obesity, higher HOMA-IR, systolic blood pressure and C-reactive protein. Belonging to the highest pro-NT quartile correlated with greater leptin levels independent of age, gender and other confounders (r2=0.82, p=0.02).CONCLUSIONS: Circulating leptin is associated with higher proNT levels independent of diabetes, obesity and metabolic syndrome components; besides its effects on central leptin signaling, NT may influence energy balance by modulating circulating leptin concentration likely through a mechanism involving gut fat absorption.