Cholinergic and nitrergic regulation of in vivo giant migrating contractions in rat colon
Cholinergic and nitrergic regulation of in vivo giant migrating contractions in rat colon
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DOI：
10.1152/ajpgi.00114.2001
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发表时间：
2002-09-01
影响因子：
4.5
通讯作者：
Sarna, SK
中科院分区：
文献类型：
--
作者：
Li, M;Johnson, CP;Sarna, SK
本研究的目的是表征在正常和炎症状态下的大鼠结肠运动活性，并确定其神经调节。通过手术植入的应变传感器记录环形肌肉收缩。大鼠结肠主要表现出巨大的迁移性收缩（GMCs），其频率在远端降低。只有一小部分GMCs向远端方向传播，其余的则是随机发生的。相性收缩存在，但其幅度是非常小的GMCs相比。口服葡聚糖硫酸钠诱导的炎症抑制了近端和中部的GMC频率，但在远端结肠中没有。腹腔注射阿托品和4-二苯基乙酰氧基-N-甲基-哌啶碘甲烷可抑制GMC的频率，而N-w-硝基-L-精氨酸甲酯可增强GMC的频率。5-羟色胺、速激肽、降钙素基因相关肽受体或受体亚型拮抗剂以及乙啶和苏拉明对GMCs的频率无显著影响。维拉帕米短暂抑制GMC。总之，与犬和人类结肠不同，大鼠结肠表现出频繁的GMC，并且它们的频率在炎症中受到抑制。在体内，GMC通过作用于M-3受体的乙酰胆碱的神经释放来刺激。一氧化氮的组成性释放可部分抑制其频率。
The aim of this study was to characterize in vivo rat colonic motor activity in normal and inflamed states and determine its neural regulation. Circular muscle contractions were recorded by surgically implanted strain-gauge transducers. The rat colon exhibited predominantly giant migrating contractions (GMCs) whose frequency decreased distally. Only a small percentage of these GMCs propagated in the distal direction; the rest occurred randomly. Phasic contractions were present, but their amplitude was very small compared with that of GMCs. Inflammation induced by oral administration of dextran sodium sulfate suppressed the frequency of GMCs in the proximal and middle but not in the distal colon. Frequency of GMCs was suppressed by intraperitoneally administered atropine and 4-diphenylacetoxy-N-methyl-piperidine methiodide and was enhanced by N-w-nitro-L-arginine methyl ester. Serotonin, tachykinin, and calcitonin gene-related peptide receptor or receptor subtype antagonists as well as guanethidine and suramin had no significant effect on the frequency of GMCs. Verapamil transiently suppressed the GMCs. In conclusion, unlike the canine and human colons, the rat colon exhibits frequent GMCs and their frequency is suppressed in inflammation. In vivo GMCs are stimulated by neural release of acetylcholine that acts on M-3 receptors. Constitutive release of nitric oxide may partially suppress their frequency.