5-Ethynyl-2'-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by "click" chemistry
5-Ethynyl-2'-deoxyuridine as a molecular probe of cell proliferation for high-content siRNA screening assay by "click" chemistry
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DOI：
10.1007/s11426-011-4398-4
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发表时间：
2011-11-01
影响因子：
9.6
通讯作者：
Zhang BiLiang
中科院分区：
文献类型：
--
作者：
Chen MiaoJuan;Qu DeZhong;Zhang BiLiang
增殖细胞的标记和鉴定对于高含量筛选（HCS）测定中的生理或病理过程的研究是重要的。在这里，我们描述了乙炔基脱氧尿苷（EdU）作为生物标志物的细胞增殖的评估，并清楚地证明了HCS测定中使用的EdU标记方法的可行性。EdU检测是高度稳健的、可重复的、技术简单的，并且非常适合于自动化分割，这为建立siRNA、miRNA和小分子文库的多重HCS测定提供了极好的替代方案。
Labelling and identification of proliferating cells is important for the study of physiological or pathological processes in high-content screening (HCS) assays. Here we describe ethynyl deoxyuridine (EdU) as a biomarker for the assessment of cell proliferation and clearly demonstrate the feasibility of the EdU-labelling method for use in HCS assays. EdU detection is highly robust, reproducible, technically simple, and well suited for automated segmentation, which provides an excellent alternative for setting up multiplexed HCS assays of siRNA, miRNA and small-molecule libraries.