Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter
Cloning of STK13, a third human protein kinase related to Drosophila aurora and budding yeast Ipl1 that maps on chromosome 19q13.3-ter
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DOI：
10.1006/geno.1998.5522
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发表时间：
1998-11-01
期刊：
影响因子：
4.4
通讯作者：
Prigent, C
中科院分区：
文献类型：
--
作者：
Bernard, M;Sanseau, P;Prigent, C
本报告描述了编码 STK13 的 cDNA 的鉴定，STK13 是与果蝇 Aurora 和芽殖酵母 Ip11 激酶相关的第三种人类蛋白激酶。用编码 pEg2 蛋白激酶的非洲爪蟾 cDNA 和睾丸 mRNA 上的 5' RACE 筛选人胎盘 cDNA 文库后，分离出全长 cDNA。通过体细胞和辐射杂交组 PCR 筛选的组合，确定 STK13 在标记 D19S210 和 D19S218 之间的 19q13.3-ter 上的染色体定位。使用含有 STK13 的基因组克隆作为探针，通过荧光原位杂交 (FISH) 证实了 STK13 在人类 19 号染色体上的定位，(C) 1998 年学术出版社。
This report describes the identification of a cDNA encoding STK13, a third human protein kinase related to the Drosophila Aurora and the budding yeast Ip11 kinases. After screening of a human placental cDNA library with a Xenopus laevis cDNA encoding the pEg2 protein kinase and 5' RACE on testis mRNA, a full-length cDNA was isolated. The chromosomal localization of STK13 on 19q13.3-ter between the markers D19S210 and D19S218 was established by a combination of somatic cell and radiation hybrid panel PCR screening. The localization of STK13 on human chromosome 19 was confirmed by fluorescence in situ hybridization (FISH) using a genomic clone containing STK13 as a probe, (C) 1998 Academic Press.