Use of genetic markers for the detection of off-types for DUS phenotypic traits in the inbreeding crop, barley
Use of genetic markers for the detection of off-types for DUS phenotypic traits in the inbreeding crop, barley
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DOI：
10.1007/s11032-019-1088-y
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发表时间：
2020-01-10
影响因子：
3.1
通讯作者：
Cockram, James
中科院分区：
文献类型：
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作者：
Saccomanno, Benedetta;Wallace, Margaret;Cockram, James
作物异型的检测具有多种用途，包括在植物育种权授予（PBR）的显著性、均匀性和稳定性（DUS）测试期间对植物新品种申请的一致性进行评估。在这里，我们研究了遗传标记，在本例中是竞争性等位基因特异性PCR (KASP)，是否可以用于鉴定近交谷物作物大麦（Hordeum vulgare）中DUS表型评估的off型。为了证明原理，KASP标记诊断了9种DUS表型的表型表达，并使用了两个大麦品种（‘Pelican’和‘Felicie’）的DNA在每个标记上携带对比等位基因。我们发现，对于大多数标记，可以将等位基因健壮地调用到模板DNA浓度为2 ng，但不是
Detection of crop off-types is of interest for multiple uses, including the assessment of uniformity for new plant variety applications during distinctness, uniformity and stability (DUS) testing for the awarding of plant breeders' rights (PBR). Here, we investigate whether genetic markers, in this case Kompetitive Allele-Specific PCR (KASP), can be used for the identification off-types for phenotypes assessed for DUS in the inbreeding cereal crop, barley (Hordeum vulgare). To demonstrate proof of principle, KASP markers diagnostic for phenotypic expression of nine DUS phenotypes, and DNA from two barley varieties ('Pelican' and 'Felicie') carrying contrasting alleles at each marker were used. We found that for the majority of markers, it was possible to robustly call alleles down to template DNA concentrations of 2 ng, but not