The effects of maturation and aging on the rotator cuff tendon-to-bone interface.
The effects of maturation and aging on the rotator cuff tendon-to-bone interface.
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DOI：
10.1096/fj.202101484r
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发表时间：
2021-12
期刊：
影响因子：
--
通讯作者：
Duan B
中科院分区：
文献类型：
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作者：
Jiang X;Wojtkiewicz M;Patwardhan C;Greer S;Kong Y;Kuss M;Huang X;Liao J;Lu Y;Dudley A;Gundry RL;Fuchs M;Streubel P;Duan B
肩袖肌腱损伤通常发生在肌腱与骨的界面（即附着点）区域，在老年人群中发病率较高，但这种现象的根本原因仍不清楚。本研究的目的是确定小鼠模型中肩袖附着点随着成熟和衰老而发生的组织学、分子和生物力学变化。研究了四个不同年龄组的小鼠（新生、幼年、成年和老年）。新生儿组和其他成熟组的附着点存在显着差异，新生儿组的胶原含量、蛋白聚糖沉积、胶原纤维分散度显着较高。年轻组、成年组和老年组的成分和组织学特征没有表现出显着差异，除了老年组插入部位的蛋白聚糖沉积增加和胶原纤维变薄之外。纳米压痕测试表明，与其他组相比，旧组在插入部位的压缩模量更小。然而，拉伸机械测试表明，与年轻组和成年组相比，老年组表现出显着更高的失效应力。蛋白质组学分析发现，新生儿和年轻群体之间的蛋白质含量存在显着差异，但年轻群体、成人和老年群体之间的变化较小。这些结果表明：（1）从新生儿到幼儿时期，附着点的组成和结构发生了显着的变化； (2)老年人群中肩袖肌腱损伤的风险增加不仅仅因为啮齿动物模型中的老年。
Rotator cuff tendon injuries often occur at the tendon-to-bone interface (i.e., enthesis) area, with a high prevalence for the elderly population, but the underlying reason for this phenomenon is still unknown. The objective of this study is to identify the histological, molecular, and biomechanical alterations of the rotator cuff enthesis with maturation and aging in a mouse model. Four different age groups of mice (newborn, young, adult, and old) were studied. Striking variations of the entheses were observed between newborn and other matured groups, with collagen content, proteoglycan deposition, collagen fiber dispersion significantly higher in the newborn group. The compositional and histological features of young, adult, and old groups did not show significant differences, except having increased proteoglycan deposition and thinner collagen fibers at the insertion sites in the old group. Nanoindentation testing showed that the old group had a smaller compressive modulus at the insertion site when compared with other groups. However, tensile mechanical testing reported that the old group demonstrated a significantly higher failure stress when compared with the young and adult groups. The proteomics analysis detected dramatic differences in protein content between newborn and young groups but minor changes among young, adult, and old groups. These results demonstrated: (1) the significant alterations of the enthesis composition and structure occur from the newborn to the young time period; (2) the increased risk of rotator cuff tendon injuries in the elderly population is not solely because of old age alone in the rodent model.