Polystyrene microspheres as a specific marker for the diagnosis of aspiration in hamsters
Polystyrene microspheres as a specific marker for the diagnosis of aspiration in hamsters
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DOI：
10.1165/rcmb.2002-0028oc
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发表时间：
2002-10-01
影响因子：
6.4
通讯作者：
Springer, C
中科院分区：
文献类型：
--
作者：
Avital, A;Shapiro, E;Springer, C
幼儿反复吸食的诊断是有问题的，因为没有特定的金标准可供使用。将生理盐水或白色聚苯乙烯微球悬浮液注入金黄地鼠气管内(n=42)，观察90 d后的细胞学、微球指数(微球总数/100个巨噬细胞)和肺组织学变化。分别于气管注入后第1、3、10、32、60、90d处死动物(生理盐水灌注组3只，微球灌注组4只)，行BAL。气管内滴注生理盐水或微球混悬液后第1天，BAL出现明显的炎症反应。在研究的所有日子里，肺泡巨噬细胞内都清楚地识别出白色微球。微球数量显示10d后50%的消失率。肺组织学仅在滴注后第1天观察到轻度的支气管周炎症。肺外器官中未检测到微球。我们的结论是，注入金黄地鼠气管的聚苯乙烯微球在BAL巨噬细胞中可以很容易地识别长达3个月，并有可能作为一种敏感、特异和稳定的诊断吸入性疾病的标志物。
The diagnosis of recurrent aspiration in young children is problematic because there is no specific gold standard test to be used. In the present work, normal saline or a suspension of white polystyrene microspheres in normal saline was instilled into hamsters' trachea (n = 42), and bronchoalveolar lavage (BAL) cytology, microsphere index (total microspheres/100 macrophages), and lung histology were followed for 90 d. Naive animals (n = 6) had no tracheal instillation. On Days 1, 3, 10, 32, 60, and 90 after tracheal instillation, animals were killed (saline-instilled animals, n = 3; and microsphere-instilled animals, n = 4), and BAL was performed. There was a marked inflammatory response in BAL on Day 1 after tracheal instillation of saline or microsphere suspension. White microspheres were clearly identified within alveolar macrophages in all studied days. Microsphere numbers showed a 50% disappearance rate of 10 d. A mild peribronchial inflammation was noted in lung histology only on Day 1 after instillation. Microspheres were not detected in extrapulmonary organs. We conclude that polystyrene microspheres instilled in hamsters' trachea can be easily identified in BAL macrophages for as long as 3 mo and could potentially be used as a sensitive, specific, and stable marker for the diagnosis of aspiration.