Candida albicans Isolates 529L and CHN1 Exhibit Stable Colonization of the Murine Gastrointestinal Tract.
Candida albicans Isolates 529L and CHN1 Exhibit Stable Colonization of the Murine Gastrointestinal Tract.
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DOI：
10.1128/mbio.02878-21
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发表时间：
2021-12-21
期刊：
影响因子：
6.4
通讯作者：
Ene IV
中科院分区：
文献类型：
--
作者：
McDonough LD;Mishra AA;Tosini N;Kakade P;Penumutchu S;Liang SH;Maufrais C;Zhai B;Taur Y;Belenky P;Bennett RJ;Hohl TM;Koh AY;Ene IV
白色念珠菌是一种病原体，定植在人体的多个生态位，包括胃肠道，但也负责粘膜和全身感染。尽管白色念珠菌普遍存在于人类共生体中，但小鼠胃肠道通常难以定植白色念珠菌参考分离物SC5314。在这里，我们鉴定了两株白色念珠菌，529L和CHN1，它们在三种不同的动物设施中稳定地定植在小鼠胃肠道中，而SC5314从该生态位中丢失。细菌微生物群的分析没有显示出三种白色念珠菌菌株定殖的小鼠之间的显着差异。我们比较了这三种菌株的基因型和表型，并鉴定了数千个单核苷酸多态性（snp）和多种表型差异，包括它们对营养线索的生长和丝化能力。然而，尽管在实验室条件下存在显著的丝化差异，但对胃肠道细胞形态的分析表明，这三种分离株在体内生态位中表现出相似的丝化特性。值得注意的是，我们发现SC5314对抗菌肽CRAMP更敏感，与CHN1和529L相比，使用CRAMP缺陷小鼠适度增加了SC5314在胃肠道定植的能力。这些研究为菌株特异性差异如何影响宿主白色念珠菌的性状提供了新的见解，并推动了CHN1和529L作为相关菌株在其自然宿主生态位中研究白色念珠菌的病理生物学。
Candida albicans is a pathobiont that colonizes multiple niches in the body including the gastrointestinal (GI) tract but is also responsible for both mucosal and systemic infections. Despite its prevalence as a human commensal, the murine GI tract is generally refractory to colonization with the C. albicans reference isolate SC5314. Here, we identify two C. albicans isolates, 529L and CHN1, that stably colonize the murine GI tract in three different animal facilities under conditions where SC5314 is lost from this niche. Analysis of the bacterial microbiota did not show notable differences among mice colonized with the three C. albicans strains. We compared the genotypes and phenotypes of these three strains and identified thousands of single nucleotide polymorphisms (SNPs) and multiple phenotypic differences, including their ability to grow and filament in response to nutritional cues. Despite striking filamentation differences under laboratory conditions, however, analysis of cell morphology in the GI tract revealed that the three isolates exhibited similar filamentation properties in this in vivo niche. Notably, we found that SC5314 is more sensitive to the antimicrobial peptide CRAMP, and the use of CRAMP-deficient mice modestly increased the ability of SC5314 to colonize the GI tract relative to CHN1 and 529L. These studies provide new insights into how strain-specific differences impact C. albicans traits in the host and advance CHN1 and 529L as relevant strains to study C. albicans pathobiology in its natural host niche.
DOI：
10.1093/g3journal/jkab110
发表时间：
2021-07-14
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Thomson GJ;Kakade P;Hirakawa MP;Ene IV;Bennett RJ
通讯作者：
Bennett RJ