PCR-based methods to the diagnosis of imported malaria
PCR-based methods to the diagnosis of imported malaria
复制标题
DOI：
10.1051/parasite/2008153484
复制
发表时间：
2008-09-01
期刊：
影响因子：
2.9
通讯作者：
Magnaval, J. F.
中科院分区：
文献类型：
--
作者：
Berry, A.;Benoit-Vical, F.;Magnaval, J. F.
疟疾的快速、准确诊断对于从流行区归来的发热病人来说是非常必要的，自从首次描述了用聚合酶链式反应(PCR)诊断疟原虫感染以来，这种分子方法在输入性疟疾实验室诊断中的作用仍然是一个热门的问题。基于聚合酶链式反应的检测被发现比所有常规方法更敏感和更特异，分子诊断的最大贡献是聚合酶链式反应阴性的结果将确定没有任何疟疾感染，从而迅速将调查定向到其他病原学。这项技术现在应被视为诊断输入性疟疾的金标准。
Rapid and precise diagnosis of malaria is needed to take care febrile patient returning from endemic areas, Since the first description of the diagnosis of Plasmodium infection by polymerase-chain-reaction (PCR), the role of this kind of molecular method in the laboratory diagnosis of imported malaria is still a topical question. PCR-based assays were found to be more sensitive and more specific than all conventional methods, The highest contribution of the molecular diagnosis is that a PCR negative result would ascertain the lack of any malaria infection, thus quickly orienting the investigations toward other aetiology. This technique should be now considered as the gold standard for the diagnosis of imported malaria.