The effect of different desolvating agents on BSA nanoparticle properties and encapsulation of curcumin
The effect of different desolvating agents on BSA nanoparticle properties and encapsulation of curcumin
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DOI：
10.1007/s11051-014-2565-1
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发表时间：
2014-08-29
影响因子：
2.5
通讯作者：
Kokini, J.
中科院分区：
文献类型：
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作者：
Sadeghi, R.;Moosavi-Movahedi, A. A.;Kokini, J.
以牛血清白蛋白（BSA）为原料，分别以乙醇、丙酮及其混合物（70：30和50：50）为溶剂，采用去溶剂化法制备了纳米粒。乙醇以及乙醇和丙酮的混合物导致最球形的纳米颗粒，而使用纯丙酮导致球形和棒状纳米颗粒的混合物。丙酮是具有较高包封效率的溶剂，等于99.2 +/-0.36%。对于乙醇（100）4x、Et：Ac（70：30）4x、Et：Ac（50：50）4x和丙酮（100）3x，本研究中BSA NP的多分散性值分别为0.045 +/- 0.007、0.065 +/- 0.013、0.091 +/- 0.012和0.120 +/- 0.016。姜黄素与BSA的摩尔比为1.0和1.5时，姜黄素在BSA NP内的包封效率分别为19.4 +/-2.2和19.8 +/-1.6%。使用戊二醛交联提高了BSA纳米颗粒和姜黄素负载的BSA纳米颗粒的稳定性，并且两组纳米颗粒均稳定1个月;冻干的姜黄素负载的BSA纳米颗粒能够再分散在水中。再分散的纳米颗粒的粒径和多分散指数高于冻干前的原始纳米颗粒。尺寸分布研究表明，在10 s的超声处理后，大多数纳米颗粒分散良好;然而，一小部分但显著的部分形成聚集体。超声处理10 s降低了再分散纳米颗粒的有效直径和多分散性，而将超声处理时间增加到20 s并未显示出显著变化。体外释放研究表明，这些生物相容性纳米粒子有能力作为载体，以提高姜黄素的控制释放。
The desolvation method was successfully used to prepare nanoparticles from bovine serum albumin (BSA) using ethanol, acetone, and their mixtures (70:30 and 50:50, respectively). Ethanol and mixtures of ethanol and acetone led to the most spherical nanoparticles, while using pure acetone resulted in a mixture of spherical and rod shape nanoparticle. Acetone was the solvent with higher encapsulation efficiency equal to 99.2 +/- 0.36 %. The polydispersity values of BSA NPs in this study were 0.045 +/- 0.007, 0.065 +/- 0.013, 0.091 +/- 0.012, and 0.120 +/- 0.016 for ethanol (100) 4x, Et:Ac (70:30) 4x, Et:Ac (50:50) 4x, and acetone (100) 3x, respectively. Encapsulation efficiencies of curcumin inside BSA NPs were 19.4 +/- 2.2 and 19.8 +/- 1.6 % for 1.0 and 1.5 molar ratios of curcumin to BSA, respectively. Crosslinking using glutaraldehyde improved the stability of BSA NPs and curcumin-loaded BSA NPs and both groups of nanoparticles were stable for 1 month; the lyophilized curcumin-loaded BSA NPs were able to redisperse in water. The particle size and polydispersity index of redispersed NPs were higher than the original NPs before lyophilization. The size distribution study shows that after 10 s of sonication most nanoparticles were well dispersed; however, a small but significant fraction formed aggregates. Sonication for 10 s decreased the effective diameter and polydispersity of the redispersed nanoparticles, while increasing the sonication time to 20 s did not show significant changes. In vitro release study of curcumin from BSA NPs showed that these biocompatible nanoparticles have the ability to be used as a carrier to improve controlled release of curcumin.