Zinc induces cell cycle arrest and apoptosis by upregulation of WIG-1 in esophageal squamous cancer cell line EC109
Zinc induces cell cycle arrest and apoptosis by upregulation of WIG-1 in esophageal squamous cancer cell line EC109
复制标题
锌通过上调食管鳞癌细胞系 EC109 中的 WIG-1 诱导细胞周期停滞和细胞凋亡
DOI：
10.1007/s13277-011-0182-5
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发表时间：
2011-08-01
期刊：
影响因子：
--
通讯作者：
Ma, Zheng
中科院分区：
文献类型：
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作者：
Guo, Wei;Zou, Ying-Bo;Ma, Zheng
锌缺乏与人类食管鳞状细胞癌（ESCC）的病因有关。从人3q26.3区克隆出锌指蛋白野生型p53诱导基因1 (WIG-1)，编码289个氨基酸的多肽。我们之前的研究表明，wigg -1在ESCC组织中表达下调。本实验研究了锌对EC109细胞增殖、凋亡及wig -1表达的影响。同时，将wig -1的RNAi载体转染到EC109细胞中，研究锌对wig -1表达的影响。我们发现锌能抑制EC109细胞增殖，诱导G0/G1细胞周期阻滞和凋亡，这种作用可能与改变凋亡相关基因bax、p21WAF、cyclin D1的表达有关。特别是在补锌后，我们观察到wig -1表达上调，这表明wig -1可能通过调节bax、p21WAF和cyclin D1的表达参与了锌诱导的EC109细胞周期阻滞和凋亡。
Zinc deficiency was implicated in the etiologies of human esophageal squamous cell carcinoma (ESCC). Wild-type p53-induced gene 1 (WIG-1), a kind of zinc finger protein, was cloned from the human 3q26.3 region and encoded a putative polypeptide of 289 amino acids. Our previous studies have demonstrated that the expression ofWIG-1was downregulated in ESCC tissues. Herein, we investigated the effect of zinc on cell proliferation, apoptosis, as well as expression ofWIG-1in EC109 cells. Meanwhile, an RNAi vector ofWIG-1was transfected into EC109 cells and the effect of zinc onWIG-1expression was investigated. We found that zinc could suppress cell proliferation and induce G0/G1 cell cycle arrest and apoptosis of EC109, and this efficacy might result from the expression altering of several apoptosis-related genes, such asBax,p21WAF, andcyclin D1. In particular, upregulation ofWIG-1was observed after zinc supplementation, indicating thatWIG-1might be involved in the zinc-induced cell cycle arrest and apoptosis of EC109 cells by regulating the expression ofBax,p21WAF, andcyclin D1.