Microsomal triglyceride transfer protein gene expression and triglyceride accumulation in hypoxic human hearts
Microsomal triglyceride transfer protein gene expression and triglyceride accumulation in hypoxic human hearts
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DOI：
10.1161/01.atv.0000030199.06252.26
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发表时间：
2002-09-01
影响因子：
8.7
通讯作者：
Andersen, CB
中科院分区：
文献类型：
--
作者：
Nielsen, LB;Perko, M;Andersen, CB
目的——心肌细胞分泌载脂蛋白 (apo)B 含脂蛋白。它们的功能可能是当脂肪酸的β-氧化减少时，例如在缺氧期间，去除甘油三酯。为了检验这一假设，我们检查了接受冠状动脉旁路移植术 (CABG，n=13) 或瓣膜置换术 (n=6) 手术的患者的心脏活检。 方法和结果 - 与瓣膜置换术患者相比，CABG 患者的心室微粒体甘油三酯转移蛋白 (P=0.02) 和 apoB (P=0.04) mRNA 水平均高出约 2 倍。在CABG患者中，心室微粒体甘油三酯转移蛋白mRNA水平与心室肌细胞中甘油三酯含量（r=-0.70；P=0.02）和甾醇调节元件结合蛋白-1的mRNA水平（r=-0.74；P=0.004）呈负相关。结论-结果与心脏脂蛋白产生增加的观点相一致。 人心室缺氧，可能是由于甾醇调节元件结合蛋白 1 表达减少所致。这可能会减弱心肌细胞中甘油三酯的积累。
Objectives-Cardiac myocytes secrete apolipoprotein (apo)B-containing lipoproteins. Their function may be the removal of triglycerides when beta-oxidation of fatty acids is decreased, eg, during hypoxia. To test this hypothesis, we examined heart biopsies from patients undergoing coronary artery bypass graft (CABG, n=13) or valve replacement (n=6) surgery.Methods and Results-Ventricular microsomal triglyceride transfer protein (P=0.02) and apoB (P=0.04) mRNA levels were both approximate to2-fold higher in CABG compared with valve replacement patients. In CABG patients, ventricular microsomal triglyceride transfer protein mRNA levels were negatively associated with the triglyceride content in ventricular myocytes (r=-0.70; P=0.02) and with mRNA levels of sterol regulatory element binding protein-1 (r=-0.74; P=0.004).Conclusions-The results are compatible with the notion that cardiac lipoprotein production is increased in hypoxic human ventricle, possibly as a result of decreased sterol regulatory element binding protein-1 expression. This might attenuate accumulation of triglycerides in cardiac myocytes.