Recruitment of osteoclasts in the mandible of osteopetrotic (op/op) mice
Recruitment of osteoclasts in the mandible of osteopetrotic (op/op) mice
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DOI：
10.1111/j.0909-8836.2004.00109.x
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发表时间：
2004-04-01
影响因子：
1.9
通讯作者：
Saku, T
中科院分区：
文献类型：
--
作者：
Ida-Yonemochi, H;Ishibashi, O;Saku, T
由于巨噬细胞集落刺激因子（M-CSF）功能活性的缺失，骨石症（op/op）小鼠中的破骨细胞显着减少。然而，众所周知，随着衰老，破骨细胞会出现在op/op骨骼中，尽管其分子机制尚不清楚。为了研究op/op小鼠颌骨中破骨细胞的募集，通过抗酒石酸酸性磷酸酶活性的组织化学和组织蛋白酶K的免疫组织化学分析了出生后2年的破骨细胞分布。op/op下颌骨中的破骨细胞数量在出生后迅速减少，并在出生后3天消失，尽管出生时op/op和正常同窝小鼠之间的破骨细胞分布没有差异。 2周时，破骨细胞开始在op/op牙胚周围重新出现，牙胚和骨小梁之间没有明显的结缔组织层介入。它们的数量增加并分散在下颌骨上，在第 8 周时达到最大值，此时可以在门牙细菌周围识别出牙周膜样结构。随后破骨细胞又逐渐减少，62周后op/op下颌骨中可见少量破骨细胞，其骨髓间隙消失。这些发现表明，破骨细胞在 op/op 下颌骨中以独立于 M-CSF 的方式募集，特别是在牙胚周围的区域。
Osteoclasts in osteopetrotic (op/op) mice are substantially reduced by the absence of functional activities of macrophage colony-stimulating factor (M-CSF). However, it is known that osteoclasts appear in op/op skeletal bones with aging, although the molecular mechanism for this is unknown. In order to investigate osteoclastic recruitment in the jaw bones of op/op mice, osteoclastic distribution was analysed for 2 yr after birth by histochemistry for tartrate-resistant acid phosphatase activity and immunohistochemistry for cathepsin K. Osteoclasts in op/op mandibular bones decreased rapidly in number after birth and disappeared by 3 d, although there was no difference in the osteoclastic distribution between op/op and normal littermates at birth. At 2 wk, osteoclasts began to reappear around op/op tooth germs, where no apparent connective tissue layer intervened between tooth germs and bone trabeculae. They increased in number and were scattered over the mandible, reaching a maximum at 8 wk, when periodontal ligament-like structures were recognizable around incisor germs. Osteoclasts then again decreased gradually, and after 62 wk few osteoclasts were seen in op/op mandibular bones, whose marrow space disappeared. These findings suggest that osteoclasts are recruited in an M-CSF-independent manner in op/op mandibles, especially in areas around tooth germs.