Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq
Serological and Molecular Phylogenetic Detection of Coxiella burnetii in Lactating Cows, Iraq
复制标题
伊拉克泌乳牛伯氏柯克斯体的血清学和分子系统发育检测
DOI：
--
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
A. Yousif
中科院分区：
文献类型：
--
作者：
H. Gharban;A. Yousif
本研究旨在调查贝氏柯克斯体（Coxiella burnetii，C.贝氏体）感染的牛使用酶联免疫吸附试验（ELISA）和聚合酶链反应（PCR）测定靶向IS 1111 A转座酶基因。从伊拉克瓦西特省的不同地区随机选择了共计130头泌乳奶牛，并在2018年11月至2019年5月期间进行了血液和乳汁采样。ELISA和PCR检测阳性率分别为16.15%和10%。阳性结果与临床资料有显著相关性（P<0.05）。对2个阳性PCR产物进行了遗传学分析，命名为C. burnetii IQ No.5和C.贝氏菌IQ-6号;然后在美国国家生物技术信息中心（NCBI）以登录号MN 473204.1和MN 473205.1登记。与NCBI-BLAST菌株/分离株的本地菌株的比较同一性显示97%的相似性和0.1-0.6%的总基因突变/变化。NCBI-BLAST同源序列分析表明，中国、日本、韩国、韩国、日本、韩国、日本、韩国burnetii IQ No.5和C. burnetii IQ-No.6菌株和C.贝氏体3345937（CP014354.1）荷兰分离株的分离率分别为99.10%和99.06%。目前的研究结论是，感染考克斯氏菌病的奶牛比例相对较高，应将考克斯氏菌列为流产病原体。因此，应进行包括不同动物、样本和地区的额外研究
This study is carried out to investigate the prevalence of Coxiella burnetii (C. burnetii) infections in cattle using an enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) assay targeting IS1111A transposase gene. A total of 130 lactating cows were randomly selected from different areas in Wasit province, Iraq and subjected to blood and milk sampling during the period extended between November 2018 and May 2019. ELISA and PCR tests revealed that 16.15% and 10% of the animals studied were respectively positive. Significant correlations (P<0.05) were detected between the positive results and clinical data. Two positive PCR products were analyzed phylogenetically, named as C. burnetii IQ-No.5 and C. burnetii IQ-No.6; and then recorded in the National Center for Biotechnology Information (NCBI) under an accession numbers of MN473204.1 and MN473205.1. Comparative identity of the local strains with NCBI-BLAST strains/isolates revealed 97% similarity and 0.1-0.6% of total genetic mutations/changes. NCBI-BLAST Homology Sequence reported high significant identity (P<0.05) between the local, C. burnetii IQ-No.5 and C. burnetii IQ-No.6; strains and C. burnetii 3345937 (CP014354.1) Netherlands isolate at 99.10% and 99.06%, respectively. The current study concluded that the percentage of infected cows with coxiellosis is relatively high, and Coxiella should be listed as abortive pathogen. Therefore, additional studies should be performed including different animals, samples, and regions