PhyResSE: a Web Tool Delineating Mycobacterium tuberculosis Antibiotic Resistance and Lineage from Whole-Genome Sequencing Data
PhyResSE: a Web Tool Delineating Mycobacterium tuberculosis Antibiotic Resistance and Lineage from Whole-Genome Sequencing Data
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DOI：
10.1128/jcm.00025-15
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发表时间：
2015-06-01
影响因子：
9.4
通讯作者：
Fellenberg, Kurt
中科院分区：
文献类型：
--
作者：
Feuerriegel, Silke;Schleusener, Viola;Fellenberg, Kurt
抗生素耐药性结核病构成全球威胁，每年导致数十万人死亡。虽然全基因组测序 (WGS) 以其前所未有的详细程度有望在诊断中发挥越来越重要的作用，但数据分析是一项艰巨的挑战。在这里，我们提供了一个简单易用的 Web 服务（免费供学术使用，网址为 http://phyresse.org）。它描述了谱系和耐药性，为未经生物信息学培训的生命科学家和医生提供了最先进的方法。它结合了适合人类诊断的精细数据处理和质量控制，以及从内部和全球范围内的特征良好的样本中收集的经过验证的耐药性数据宝库。
Antibiotic-resistant tuberculosis poses a global threat, causing the deaths of hundreds of thousands of people annually. While whole-genome sequencing (WGS), with its unprecedented level of detail, promises to play an increasingly important role in diagnosis, data analysis is a daunting challenge. Here, we present a simple-to-use web service (free for academic use at http://phyresse.org). Delineating both lineage and resistance, it provides state-of-the-art methodology to life scientists and physicians untrained in bioinformatics. It combines elaborate data processing and quality control, as befits human diagnostics, with a treasure trove of validated resistance data collected from well-characterized samples in-house and worldwide.