Afuni, a novel transforming growth factor-β gene is involved in arm regeneration by the brittle star Amphiura filiformis
Afuni, a novel transforming growth factor-β gene is involved in arm regeneration by the brittle star Amphiura filiformis
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DOI：
10.1007/s00427-005-0487-8
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发表时间：
2005-08-01
影响因子：
2.4
通讯作者：
Beesley, PW
中科院分区：
文献类型：
--
作者：
Bannister, R;McGonnell, IM;Beesley, PW
骨形态发生蛋白（BMPs）是转化生长因子- β (tgf - β)超家族的一个家族，在脊椎动物和无脊椎动物的发育过程中发挥多种作用。在这里，我们报道了一种新的BMP的分子克隆从蛇形文昌鱼再生臂。这种新型BMP的理论翻译氨基酸序列与海胆BMP univin有很高的相似性。这种新颖的BMP被命名为afuni。全载原位杂交与手臂再生有关。表达发生在晚期再生（消融后3周和5周）的不同近端和远端区域。这些位点处于再生的不同阶段，表明该基因在成人手臂发育中具有多种作用。该基因的细胞表达发生在再生和非再生臂径向水渠（RWC）内的迁移细胞中。这些迁移的体腔细胞表明，体腔RWC作为一种细胞材料的来源，在线虫手臂再生中起着关键作用。
The bone morphogenetic proteins (BMPs) are a family of the transforming growth factor-beta (TGF-beta) superfamily that perform multiple roles during vertebrate and invertebrate development. Here, we report the molecular cloning of a novel BMP from regenerating arms of the ophiuroid Amphiura filiformis. The theoretically translated amino acid sequence of this novel BMP has high similarity to that of the sea urchin BMP univin. This novel BMP has been named afuni. Whole-mount in situ hybridisation implicates afuni in arm regeneration. Expression occurs in distinct proximal and distal regions of late regenerates (3- and 5-week postablation). These sites are at different stages of regeneration, suggesting multiple roles for this gene in adult arm development. Cellular expression of this gene occurs in migratory cells within the radial water canal (RWC) of regenerating and nonregenerating arms. These migrating coelomocytes suggest a key role for the coelomic RWC as a source of the cellular material for use in arm regeneration by A. filiformis.