Interaction Proteomics Reveals Brain Region-Specific AMPA Receptor Complexes
Interaction Proteomics Reveals Brain Region-Specific AMPA Receptor Complexes
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DOI：
10.1021/pr500697b
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发表时间：
2014-12-01
影响因子：
4.4
通讯作者：
Li, Ka Wan
中科院分区：
文献类型：
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作者：
Chen, Ning;Pandya, Nikhil J.;Li, Ka Wan
大脑中的快速兴奋性突触传播是由作用于突触后AMPA受体的谷氨酸介导的。最近的研究揭示了大量AMPA受体辅助蛋白，这可能有助于调节AMPA受体运输，亚细胞受体定位和受体门控性能。在这里，我们通过全面的相互作用蛋白质组学研究了皮质，海马和小脑的AMPA受体相互作用。该研究表明，AMPA受体辅助蛋白参与不同大脑区域特异性AMPA受体亚复合体，这可能是AMPA受体特性的大脑区域特异性调节。根据大脑区域的不同，相互作用的蛋白可能与AMPA和非AMPA受体复合物有关。
Fast excitatory synaptic transmission in the brain is mediated by glutamate acting on postsynaptic AMPA receptors. Recent studies have revealed a substantial number of AMPA receptor auxiliary proteins, which potentially contribute to the regulation of AMPA receptor trafficking, subcellular receptor localization, and receptor gating properties. Here we examined the AMPA receptor interactomes from cortex, hippocampus, and cerebellum by comprehensive interaction proteomics. The study reveals that AMPA receptor auxiliary proteins are engaged in distinct brain region-specific AMPA receptors subcomplexes, which might underlie brain region-specific differential regulation of AMPA receptor properties. Depending on the brain region, an interacting protein can be involved in an AMPA and a non-AMPA receptor complex.