HLA-DR,DQ NUCLEOTIDE-SEQUENCE POLYMORPHISMS IN 5 MELANESIAN POPULATIONS
HLA-DR,DQ NUCLEOTIDE-SEQUENCE POLYMORPHISMS IN 5 MELANESIAN POPULATIONS
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DOI：
10.1111/j.1399-0039.1992.tb01954.x
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发表时间：
1992-07-01
期刊：
影响因子：
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通讯作者：
SERJEANTSON, SW
中科院分区：
文献类型：
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作者：
GAO, X;BHATIA, K;SERJEANTSON, SW
对来自巴布亚新几内亚海岸（马当）、岛屿（拉包尔）和高地（戈罗卡）以及新喀里多尼亚和斐济的304名美拉尼西亚人进行了HLA-DRB 1核苷酸序列多态性检查。共20个HLA-DRB 1等位基因检测外显子2 HLA-DRB 1聚合酶链反应产物的寡核苷酸杂交，在分型方案，旨在检测所有42个正式指定的HLA-DRB 1等位基因。DRB 1 *1502和1101等位基因在沿海和岛屿美拉尼西亚人中最常见，而DRB 1 *1501，1502和1408在巴布亚新几内亚高地人中占主导地位。未定义的混合淋巴细胞反应的决定因素在早期的研究美拉尼西亚人可以占在本研究中的DRB 1 *0410，1407和1408在巴布亚新几内亚高地人和DRB 1 *1104和1602在沿海地区的人。在HLA-DQA 1，-DQB 1，-DRB 3和-DRB 5的核苷酸序列多态性也被确定用于估计HLA-DR，DQ等位基因不平衡关系;在美拉尼西亚人中不常见的单倍型包括DBR 1 *1502，DRB 5 *0101和DRB 1 *0410，DQB 1 *0402。以前声称在美拉尼西亚人的HLA-DR等位基因库中存在有限的异质性，现在被认为反映了早期分型试剂的局限性，而不是HLA-DR等位基因多样性的任何显着限制。
HLA-DRB1 nucleotide sequence polymorphisms have been examined in 304 Melanesians from the Papua New Guinean coast (Madang), islands (Rabaul) and highlands (Goroka), and from New Caledonia and Fiji. A total of 20 HLA-DRB1 alleles were detected by oligonucleotide hybridizations of exon 2 HLA-DRB1 polymerase chain reaction products, in a typing protocol designed to detect all 42 officially-designated HLA-DRB1 alleles. DRB1*1502 and 1101 alleles were the most common alleles in coastal and island Melanesians, while DRB1*1501, 1502 and 1408 predominated in Papua New Guinean highlanders. Undefined mixed lymphocyte reaction determinants in earlier studies of Melanesians could be accounted for in the present study as DRB1*0410, 1407 and 1408 in Papua New Guinean highlanders and as DRB1*1104 and 1602 in coastal people. Nucleotide sequence polymorphisms at HLA-DQA1, -DQB1, -DRB3 and -DRB5 were also determined for estimating HLA-DR,DQ allelic disequilibrium relationships; unusual haplotypes in Melanesians included DBR1*1502, DRB5*0101 and DRB1*0410, DQB1*0402. Previous claims of limited heterogeneity in the HLA-DR allele repertoire in Melanesians are now seen to reflect limitations of early typing reagents rather than any dramatic restriction in HLA-DR allelic diversity.