Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases
Potential of a new strain of Bacillus amyloliquefaciens BUZ-14 as a biocontrol agent of postharvest fruit diseases
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DOI：
10.1016/j.fm.2016.11.004
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发表时间：
2017-05-01
影响因子：
5.3
通讯作者：
Venturini, M. E.
中科院分区：
文献类型：
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作者：
Calvo, H.;Marco, P.;Venturini, M. E.
以橙子、苹果、葡萄和核果为试材，研究了解淀粉芽孢杆菌BUZ-14对主要采后病害的生防效果。测定了菌株BUZ-14的温度和pH生长曲线，并测定了其对番茄灰霉病菌、苹果褐腐病菌、苹果褐腐病菌的体外抑菌活性。拉克萨、指状青霉、扩展青霉和意大利青霉。随后，通过用细胞、内生孢子和无细胞上清液处理水果来测试针对这些病原体的体内活性。体外试验结果表明，BUZ-14抑制了所有测试的病原体的生长，对应于最不敏感的物种意大利青霉和最敏感的物种M。拉克萨。体内试验证实了这些结果，因为大多数处理将核果褐腐病的发病率从100%降低到0%，确定10（7）CFU mL（-1）为最低抑制浓度。对于青霉属物种，预防性处理抑制柑橘中的指状青霉和意大利青霉的生长，并将苹果中的扩展青霉的发病率从100%降低至20%。最后，已经证明BUZ-14能够存活并控制在低温下储存的桃中的褐腐病，使其成为在园艺产品的收获后储存和销售期间应用的非常合适的生物控制剂。(C)2016爱思唯尔有限公司版权所有
The biocontrol potential of the Bacillus amyloliquefaciens strain BUZ-14 was tested against the main postharvest diseases of orange, apple, grape and stone fruit. After characterizing the temperature and pH growth curves of strain BUZ-14, its in vitro antifungal activity was determined against Botrytis cinerea, Monilinia fructicola, M. laxa, Penicillium digitatum, P. expansum and P. italicum. Subsequently, in vivo activity was tested against these pathogens by treating fruit with cells, endospores and cell-free supernatants. The in vitro results showed that BUZ-14 inhibited the growth of all the pathogens tested corresponding to the least susceptible species, P. italicum, and the most susceptible, M. laxa. In vivo tests corroborated these results as most of the treatments decreased the incidence of brown rot in stone fruit from 100% to 0%, establishing 10(7) CFU mL(-1) as the minimum inhibitory concentration. For the Penicillium species a preventive treatment inhibited P. digitatum and P. italicum growth in oranges and reduced P. expansum incidence in apples from 100% to 20%. Finally, it has been demonstrated that BUZ-14 was able to survive and to control brown rot in peaches stored at cool temperatures, making it a very suitable biocontrol agent for application during the post-harvest storage and marketing of horticultural products. (C) 2016 Elsevier Ltd. All rights reserved.