Enzymatic and genetic adaptation of soleus muscle mitochondria to physical training in rats.
Enzymatic and genetic adaptation of soleus muscle mitochondria to physical training in rats.
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比目鱼肌线粒体对大鼠体能训练的酶促和遗传适应。
DOI：
10.1152/ajpendo.1994.267.3.e388
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
M. Suzuki
中科院分区：
文献类型：
--
作者：
T. Murakami;Y. Shimomura;N. Fujitsuka;N. Nakai;Satoru Sugiyama;Takayuki Ozawa;M. Sokabe;Satoshi Horai;K. Tokuyama;M. Suzuki
为了评估运动训练对慢缩肌线粒体基因表达和线粒体生物合成的影响，成年雌性Sprague-Dawley大鼠通过在电动跑步机上跑步进行3、6和12周的训练（速度25 m/min，持续时间90 min/d，5 d/wk），测定柠檬酸合酶、泛喹啉-细胞色素-c氧化还原酶、细胞色素氧化酶、线粒体细胞色素B mRNA（通过北方印迹分析）和线粒体DNA（通过狭缝印迹和南方印迹分析）在大鼠比目鱼肌中被测量。从包含细胞色素B基因编码区的人线粒体DNA的1,500 bp片段制备用于检测线粒体mRNA和DNA的DNA探针。训练3，6，和12周显着增加柠檬酸合酶（31，28，和47%，分别），泛喹啉-细胞色素C氧化还原酶（61，63，和77%，分别），细胞色素氧化酶（25，26，和32%，分别）在肌肉中的活性。肌肉细胞色素B mRNA的含量随酶活性的增加而增加。另一方面，在肌肉中的线粒体DNA浓度没有改变训练3或6周，但增加了显着训练12周后（35%的狭缝印迹分析和31%的Southern印迹分析）。这些结果表明，在慢收缩肌的氧化能力的增加，相对短期的训练是在线粒体蛋白质合成的翻译前步骤，但增加的长期训练涉及线粒体复制调节。
To evaluate the effects of physical training on mitochondrial gene expression and mitochondrial biogenesis in slow-twitch muscle, adult female Sprague-Dawley rats were trained for 3, 6, and 12 wk by running on a motor-driven treadmill (speed of 25 m/min and duration of 90 min/day, 5 days/wk), and the activities of citrate synthase, ubiquinol-cytochrome-c oxidoreductase, cytochrome oxidase, mitochondrial cytochrome b mRNA (by Northern blot analysis), and mitochondrial DNA (by slot-blot and Southern blot analyses) were measured in rat soleus muscle. A DNA probe for detection of mitochondrial mRNA and DNA was prepared from a 1,500-bp fragment of human mitochondrial DNA that included the coding region of the cytochrome b gene. Training for 3, 6, and 12 wk significantly increased the activities of citrate synthase (31, 28, and 47%, respectively), ubiquinol-cytochrome-c oxidoreductase (61, 63, and 77%, respectively), and cytochrome oxidase (25, 26, and 32%, respectively) in muscle. The concentration of cytochrome b mRNA in the muscle was proportionally elevated with the enzyme activities. On the other hand, the mitochondrial DNA concentration in the muscle was not altered by training for 3 or 6 wk but increased significantly after training for 12 wk (35% in the slot-blot analysis and 31% in the Southern blot analysis). These results suggest that an increase in the oxidative capacity of slow-twitch muscle by the relatively short-term training is regulated at the pretranslational step in mitochondrial protein synthesis but that the increase by the long-term training involves mitochondrial replication.