IMMUNOHISTOCHEMICAL EVIDENCE OF INDOLEAMINE NEURONS IN MONKEY SPINAL-CORD
IMMUNOHISTOCHEMICAL EVIDENCE OF INDOLEAMINE NEURONS IN MONKEY SPINAL-CORD
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DOI：
10.1002/cne.902060404
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发表时间：
1982-01-01
影响因子：
2.5
通讯作者：
DELANEROLLE, NC
中科院分区：
文献类型：
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作者：
LAMOTTE, CC;JOHNS, DR;DELANEROLLE, NC
本研究通过PAP（过氧化物酶-抗过氧化物酶）方法，在成年猕猴（Macaca fascicularis和M. arctoides）脊髓中鉴定出150多个细胞，这些细胞在用5-HT（5-羟色胺）抗体染色时具有免疫反应性。用5-HT-BSA（牛血清白蛋白）偶联物（用于产生抗体）和5-HT预吸收一抗阻断染色，而BSA、多巴胺、去甲肾上腺素、5-甲氧基色胺和色胺预吸收不阻断染色。将Falck-Hillarp组织荧光技术应用于研究的3只猴子中的一只的腰骶束；结果证实了5 -羟色胺能细胞具有黄色荧光和快速褪色到棕色的特征。这些免疫反应细胞的神经元身份是基于光镜和电镜形态学以及与标记的体细胞和树突接触的突触终端的存在。这些神经元大多很小（10-25 .mu）。m)，位于x层中央管的腹侧，部分细胞突起延伸至中间灰色和腹角的腹内侧区域；X层大血管周围或中央管壁周围的其他突起。细胞在颈髓中最常见（约0.33%）。脊髓长度为6-7/mm)，胸（1.5/mm）、腰椎（3/mm）和骶（2/mm）脊髓较少见。在骶脊髓背角的边缘区和胶状区也发现少量细胞。髓质-脊髓连接处切片（obex水平）显示，脊髓细胞可能是位于第四脑室周围灰色区域中缝暗壁附近的细胞群的延伸。吲哚胺类脊髓细胞可能在脊髓回路中充当中间神经元，通过血管神经支配控制脊髓血流量，或通过中央管神经支配参与脑脊液组成。
This study identifies over 150 cells in the adult macaque [Macaca fascicularis and M. arctoides] spinal cord that are immunoreactive when stained with an antibody to 5-HT [5-hydroxytryptamine] by means of the PAP [peroxidase-antiperoxidase] method. The staining was blocked by preabsorption of the primary antibody by the 5-HT-BSA [bovine serum albumin] conjugate (used to generate the antibody) and by 5-HT, while BSA, dopamine, norepinephrine, 5-methoxytryptamine and tryptamine preabsorption did not block staining. The Falck-Hillarp histofluorescence technique was applied to the lumbosacral cord from one of the 3 monkeys studied; results confirm the presence of cells with yellow fluorescence and rapid fading to brown characteristic for serotoninergic cells. The neuronal identity of these immunoreactive cells is based on light microscopic and EM morphology and the presence of synaptic terminals in contact with labeled somata and dendrites. Most of these neurons were small (10-25 .mu.m) and located ventral to the central canal in lamina X. Some processes of the cells extended into the intermediate gray and the ventromedial area of the ventral horn; other processes wrapped around large blood vessels in lamina X or around the wall of the central canal. Cells were most frequent in the cervical cord (.apprx. 6-7/mm length of cord) and less frequent in thoracic (1.5/mm), lumbar (3/mm) and sacral (2/mm) cord. A few cells were also found in the marginal and gelatinosa regions of the dorsal horn of the sacral cord. Examination of sections from the medulla-spinal cord junction (obex level) indicates that the spinal cells may be an extension of cell groups located near the raphe obscurus in the gray region around the IVth ventricle. The indolamine spinal cells may act as interneurons in spinal circuits, control spinal blood flow through vessel innervation, or play a role in CSF composition through central canal innervation.