Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis
Detection of canine distemper virus by reverse transcriptase-polymerase chain reaction in the urine of dogs with clinical signs of distemper encephalitis
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DOI：
10.1016/j.rvsc.2005.03.002
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发表时间：
2006-02-01
影响因子：
2.4
通讯作者：
Alfieri, AF
中科院分区：
文献类型：
--
作者：
Saito, TB;Alfieri, AA;Alfieri, AF
在一项前瞻性研究中，我们评估了使用尿液样本中的逆转录酶聚合酶链反应 (RT-PCR) 来诊断患有进行性神经系统疾病的狗的犬瘟热病毒。从22只犬瘟热犬的尿液中扩增出犬瘟热病毒核蛋白基因片段。 12只无症状犬的体液和白细胞RTPCR阴性。尿液样本的 RT-PCR 比血清和白细胞更敏感，并且至少与脑脊液一样敏感，可用于筛查具有神经系统症状和神经外系统症状的狗的犬瘟热。 (c) 2005 Elsevier Ltd. 保留所有权利。
In a prospective study we evaluated the use of the reverse transcriptase-polymerase chain reaction (RT-PCR) in urine samples to diagnose canine distemper virus in dogs with progressive neurological disease. A fragment of the nucleoprotein gene of canine distemper virus was amplified from the urine of 22 distemper dogs. The body fluids and leukocytes of 12 asymptomatic dogs were RTPCR negative. RT-PCR of urine samples was more sensitive than serum and leukocytes and at least as sensitive as cerebrospinal fluid to screen for distemper in dogs with neurological signs and extraneural systemic signs. (c) 2005 Elsevier Ltd. All rights reserved.